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Muscarinic Receptor Gene Transfections and In Vivo Dopamine Electrochemistry: Muscarinic Receptor Control of Dopamine-Dependent Reward and Locomotion

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Muscarinic Receptor: From Structure to Animal Models

Part of the book series: Neuromethods ((NM,volume 107))

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Abstract

Cholinergic neurons in laterodorsal (LDT) and pedunculopontine (PPT) tegmental nuclei respond to novel, arousing stimuli, then directly activate dopamine neurons, and increase dopamine outputs as measured by either in vivo microdialysis or by electrochemistry (described here). These mesopontine cholinergic neurons also directly activate superior colliculus and thalamic systems important for attention to novel stimuli, and for reward-seeking behaviors. M5 muscarinic receptors that activate dopamine neurons and reward-seeking behaviors have been studied using pharmacology, knockout mice, oligonucleotide knockdown, and with electrochemistry and Herpes simplex viral gene transfections (HSV-M5) protocols described here. Protocols for using HSV-M5 genes, and designed M4D and M3D muscarinic receptor genes in behaving mice and for dopamine electrochemistry are presented, along with consequences for drug and gene therapy.

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Acknowledgements

We thank our many collaborators and coauthors, including Gina Forster and Anthony Miller (electrochemistry), Haoran Wang, Sheena Josselyn, and Asim Rashid (HSV-M5), Jun Chul Kim and Bryan Roth (AAV-M3D and AAV-M4D), and Junichi Takeuchi, Zheng-ping Jia, John Roder, and Juergen Wess (knockouts).

Author information

Authors and Affiliations

  1. Loyola University Chicago, Chicago, IL, USA

    Stephan Steidl

  2. University of Guelph, Guelph, ON, Canada

    David Ian Wasserman

  3. University of Memphis, Memphis, TN, USA

    Charles D. Blaha

  4. Department of Psychology, University of Toronto, Sidney Smith 4002, 100 St. George Street, Toronto, ON, Canada, M5S 3G3

    John Yeomans

Authors
  1. Stephan Steidl
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  2. David Ian Wasserman
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  3. Charles D. Blaha
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  4. John Yeomans
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Corresponding author

Correspondence to John Yeomans .

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Editors and Affiliations

  1. Institute of Physiology, Charles University, Prague, Czech Republic

    Jaromir Myslivecek

  2. Department of Neurochemistry, Acad. of Sciences of the Czech Rep. Institute of Physiology, Prague, Czech Republic

    Jan Jakubik

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Steidl, S., Wasserman, D.I., Blaha, C.D., Yeomans, J. (2016). Muscarinic Receptor Gene Transfections and In Vivo Dopamine Electrochemistry: Muscarinic Receptor Control of Dopamine-Dependent Reward and Locomotion. In: Myslivecek, J., Jakubik, J. (eds) Muscarinic Receptor: From Structure to Animal Models. Neuromethods, vol 107. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2858-3_14

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  • DOI: https://doi.org/10.1007/978-1-4939-2858-3_14

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2857-6

  • Online ISBN: 978-1-4939-2858-3

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