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Localizing Proteins by Tissue Printing

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Western Blotting

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1312))

Abstract

The simple technique of making tissue prints on appropriate substrate material has made possible the easy localization of proteins, nucleic acids, carbohydrates, and small molecules in a tissue-specific mode. Plant tissues can be used to produce prints revealing a remarkable amount of anatomical detail, even without staining, which might be used to record developmental changes over time. In this chapter we will focus on the protocols for the localization of proteins and glycans using antibodies or lectins, probably the most frequently used application, but the localization of other molecules is reported and the sources indicated.

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Acknowledgment

The author is grateful to the Université Paul Sabatier (Toulouse III, France) and the CNRS for support.

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Authors and Affiliations

  1. Surfaces Cellulaires et Signalisation chez les Végétaux, UMR 5546 CNRS-Université Paul Sabatier-Toulouse III, Pôle de Biotechnologie végétale 24 Chemin de Borde Rouge BP 42617 Auzeville, 31326, Castanet-Tolosan, France

    Rafael F. Pont-Lezica Ph.D.

Authors
  1. Rafael F. Pont-Lezica Ph.D.
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Corresponding author

Correspondence to Rafael F. Pont-Lezica Ph.D. .

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Editors and Affiliations

  1. Department of Medicine, University of Oklahoma, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Department of Medicine, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Pont-Lezica, R.F. (2015). Localizing Proteins by Tissue Printing. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_13

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  • DOI: https://doi.org/10.1007/978-1-4939-2694-7_13

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2693-0

  • Online ISBN: 978-1-4939-2694-7

  • eBook Packages: Springer Protocols

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