Abstract
Protein–DNA interactions are central to many basic biological processes, including transcription regulation, DNA replication, and DNA repair. Chromatin Immunoprecipitation (ChIP) is used to determine the position and strength of protein–DNA interactions in vivo. Coupling ChIP with microarrays (ChIP-chip), and more recently with deep sequencing (ChIP-seq), has allowed genome-wide profiling of DNA binding events in vivo. In this chapter we outline the steps to generate ChIP-seq libraries from bacterial samples and briefly discuss basic analysis of the data.
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Acknowledgments
We thank Anne Stringer and Caren Stark for critical reading of the manuscript. This work is supported by NIH New Innovator Award DP2OD007188 (JTW).
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Bonocora, R.P., Wade, J.T. (2015). ChIP-Seq for Genome-Scale Analysis of Bacterial DNA-Binding Proteins. In: Artsimovitch, I., Santangelo, T. (eds) Bacterial Transcriptional Control. Methods in Molecular Biology, vol 1276. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2392-2_20
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DOI: https://doi.org/10.1007/978-1-4939-2392-2_20
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