Abstract
High-resolution multiphoton imaging of live cells has become an invaluable method to study protein dynamics in highly compartmentalized subcellular environments. Here we describe procedures that we recently developed to quantify rhodopsin mobility within and between retinal rod photoreceptor light signaling microcompartments, the disc membrane lobules, using multiphoton fluorescence relaxation after photoconversion.
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Acknowledgements
This work was supported by the National Institutes of Health grant R01EY018421 to P.D.C., a Research to Prevent Blindness, Inc. Career Development Award (P.D.C.), a grant from the Karl Kirchgessner Foundation (P.D.C.), and Lions district 20-Y1. The SUNY Upstate Department of Ophthalmology is recipient of an unrestricted grant from Research to Prevent Blindness, Inc.
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Najafi, M., Calvert, P.D. (2015). Measurements of Rhodopsin Diffusion Within Signaling Membrane Microcompartments in Live Photoreceptors. In: Jastrzebska, B. (eds) Rhodopsin. Methods in Molecular Biology, vol 1271. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2330-4_20
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DOI: https://doi.org/10.1007/978-1-4939-2330-4_20
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