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Practical Strategies for Small-Molecule Probe Development in Chemical Biology

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Chemical Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1263))

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Abstract

The effective identification, selection, and implementation of small molecules for the interrogation of biological systems require an intricate understanding of the chemical principles underlying their cellular activities. While much has been published regarding the use of screening techniques in forward chemical genetics platforms and on small-molecule target identification, less emphasis has been placed on detailed strategies for evaluating, selecting, and optimizing screening hits. This chapter provides practical tools for identifying and developing promising screening hit compounds into effective tools for biological discovery.

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Acknowledgements

We thank Gary Sulikowski for his helpful guidance and input. J.E.H. is supported by an American Heart Association Postdoctoral Fellowship (14POST19550002). C.C.H. is supported by the US NIH NHLBI (1R01HL104040), the US Veterans Administration (101BX000771), and the Cali Family Foundation.

Author information

Authors and Affiliations

  1. Department of Medicine - Cardiovascular Medicine, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN, USA

    Jonathan E. Hempel

  2. Department of Medicine - Cardiovascular Medicine, Department of Pharmacology, Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN, USA

    Charles C. Hong

Authors
  1. Jonathan E. Hempel
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  2. Charles C. Hong
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Corresponding author

Correspondence to Jonathan E. Hempel .

Editor information

Editors and Affiliations

  1. Department of Medicine - Cardiovascular Medicine, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

    Jonathan E. Hempel

  2. Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

    Charles H. Williams

  3. Department of Medicine - Cardiovascular Medicine, Department of Pharmacology, Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

    Charles C. Hong

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Hempel, J.E., Hong, C.C. (2015). Practical Strategies for Small-Molecule Probe Development in Chemical Biology. In: Hempel, J., Williams, C., Hong, C. (eds) Chemical Biology. Methods in Molecular Biology, vol 1263. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2269-7_17

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  • DOI: https://doi.org/10.1007/978-1-4939-2269-7_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2268-0

  • Online ISBN: 978-1-4939-2269-7

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