Abstract
Organismal energy homeostasis is maintained by complex interorgan communications making the discovery of novel drugs against metabolic diseases challenging using traditional high-throughput approaches in vitro. Here, we describe a method that rapidly identifies small molecules with an impact on organismal energy balance in vivo. Specifically, we developed a whole-organism screen for modulators of fasting metabolism using transgenic bioluminescence-reporter zebrafish for the gluconeogenic gene phosphoenolpyruvate-carboxykinase 1 (pck1).
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Acknowledgements
This study was supported in part by a postdoctoral fellowship DFG GU 1082/101 from the German Research Foundation to P.G., NIH grant RO1 DK60322, a pilot and feasibility award from the University of California—San Francisco diabetes center funded by NIH U01 DK089541 and the Packard Foundation to D.Y.R.S.
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Gut, P., Stainier, D.Y.R. (2015). Whole-Organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish. In: Hempel, J., Williams, C., Hong, C. (eds) Chemical Biology. Methods in Molecular Biology, vol 1263. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2269-7_12
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DOI: https://doi.org/10.1007/978-1-4939-2269-7_12
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