Abstract
Cervical cancer causes 275,000 deaths each year with 85 % of these deaths occurring in the developing world. One of the primary reasons for the concentration of deaths in developing countries is a lack of effective screening methods suited for the infrastructure of these countries. In order to address this need, we have developed a high-resolution microendoscope (HRME). The HRME is a fiber-based fluorescence microscope with subcellular resolution. Using the vital stain proflavine, we are able to image cell nuclei in vivo and evaluate metrics such as nuclear-to-cytoplasmic ratio, critical to identifying precancerous epithelial regions. In this chapter, we detail the materials and methods necessary to build this system from commercially available parts.
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References
Jemal A, Bray F, Center MM et al (2011) Global cancer statistics. CA Cancer J Clin 61:69–90
Kitchener HC, Castle PE, Cox JT (2006) Chapter 7: achievenements and limitations of cervical cytology screening. Vaccine 24S3:63–70
Papanicolaou GN, Traut HF (1941) The diagnostic value of vaginal smears in carcinoma of the uterus. Am J Obstet Gynecol 42:193–206
Sankaranarayanan R, Esmy PO, Rajkumar R et al (2007) Effect of visual screening on cervical cancer incidence and mortality in Tamil Nadu, India: a luster-randomised trial. Lancet 370(9585):398–406
Muldoon TJ, Pierce MC, Nida DL et al (2007) Subcellular-resolution molecular imaging within living tissue by fiber microendoscopy. Opt Express 15(25):16413–16423
Shin D, Pierce MC, Gillenwater AM et al (2010) A fiber-optic fluorescence microscope using a consumer-grade digital camera for in vivo cellular imaging. PLoS One 5(6):11218
Muldoon TJ, Anandasabapathy S, Maru D et al (2008) High-resolution imaging in Barrett’s esophagus: a novel, low-cost endoscopic microscope. Gastrointest Endosc 68(4):737–744
Pierce MC, Vila PM, Polydorides AD et al (2011) Low-cost endomicroscopy in the esophagus and colon. Am J Gastroenterol 106(9):1722–1724
Pierce MC, Guan Y, Quinn MK et al (2012) A pilot study of low-cost, high-resolution microendoscopy as a tool for identifying women with cervical precancer. Cancer Prev Res 5(11):1273–1279
Hitchcock CL (2011) The future of telepathology for the developing world. Arch Pathol Lab Med 135(2):211–214
Suba EJ, Murphy SK, Donelly AD et al (2006) Systems analysis of real-world obstacles to successful cervical cancer prevention in developing countries. Am J Public Health 96(3):480–487
Quinn MK, Bubi TC, Pierce MC et al (2012) High-resolution microendoscopy for the detection of cervical neoplasia in low-resource settings. PLoS One 7(9):e44924
World Health Organization (2012) Prevention of cervical cancer through screening using visual inspection with acetic acid (VIA) and treatment with cryotherapy. A demonstration project in six African countries: Malawi, Madagascar, Nigeria, Uganda, The United Republic of Tanzania, and Zambia. WHO Document Production Services
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Grant, B.D., Schwarz, R.A., Quang, T., Schmeler, K.M., Richards-Kortum, R. (2015). High-Resolution Microendoscope for the Detection of Cervical Neoplasia. In: Rasooly, A., Herold, K. (eds) Mobile Health Technologies. Methods in Molecular Biology, vol 1256. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2172-0_28
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DOI: https://doi.org/10.1007/978-1-4939-2172-0_28
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2171-3
Online ISBN: 978-1-4939-2172-0
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