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Sample Treatment Methods Involving Combinatorial Peptide Ligand Libraries for Improved Proteomes Analyses

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Clinical Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1243))

Abstract

If used in an optimized manner, the technology of combinatorial peptide solid-phase libraries can easily improve the analytical determinations of proteomes by several factors. The discovery of novel species and of early stage biomarkers becomes thus reachable with a simple sample treatment. This report describes the most important point to consider (overloading and full recovery) along with a minimum scientific background and gives then detailed recipes to laboratory technicians. Orientations for optional routes are also given according to the objective of the experimental investigations. This covers different approaches to capture proteins of very low abundance. Total protein harvestings to prevent partial losses are also described such as single exhaustive desorption and fractionated elutions for more detailed analyses. Documented results are also reported demonstrating the capability of the technology well beyond what is the common assumption.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Materials and Chemical Engineering “Giulio Natta”, Politecnico di Milano, Via Mancinelli 7, Milan, 20131, Italy

    Pier Giorgio Righetti

  2. Jam - Conseil, Paris, France

    Egisto Boschetti

Authors
  1. Pier Giorgio Righetti
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  2. Egisto Boschetti
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Corresponding author

Correspondence to Egisto Boschetti .

Editor information

Editors and Affiliations

  1. Biomedical Research Foundation, Academy of Athens, Athens, Greece

    Antonia Vlahou

  2. Biomedical Research Foundation, Academy of Athens, Athens, Greece

    Manousos Makridakis

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Righetti, P.G., Boschetti, E. (2015). Sample Treatment Methods Involving Combinatorial Peptide Ligand Libraries for Improved Proteomes Analyses. In: Vlahou, A., Makridakis, M. (eds) Clinical Proteomics. Methods in Molecular Biology, vol 1243. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1872-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-1872-0_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1871-3

  • Online ISBN: 978-1-4939-1872-0

  • eBook Packages: Springer Protocols

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