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Viral Epigenetics

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Cancer Epigenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1238))

Abstract

DNA tumor viruses including members of the polyomavirus, adenovirus, papillomavirus, and herpes virus families are presently the subject of intense interest with respect to the role that epigenetics plays in control of the virus life cycle and the transformation of a normal cell to a cancer cell. To date, these studies have primarily focused on the role of histone modification, nucleosome location, and DNA methylation in regulating the biological consequences of infection. Using a wide variety of strategies and techniques ranging from simple ChIP to ChIP-chip and ChIP-seq to identify histone modifications, nuclease digestion to genome wide next generation sequencing to identify nucleosome location, and bisulfite treatment to MeDIP to identify DNA methylation sites, the epigenetic regulation of these viruses is slowly becoming better understood. While the viruses may differ in significant ways from each other and cellular chromatin, the role of epigenetics appears to be relatively similar. Within the viral genome nucleosomes are organized for the expression of appropriate genes with relevant histone modifications particularly histone acetylation. DNA methylation occurs as part of the typical gene silencing during latent infection by herpesviruses. In the simple tumor viruses like the polyomaviruses, adenoviruses, and papillomaviruses, transformation of the cell occurs via integration of the virus genome such that the virus’s normal regulation is disrupted. This results in the unregulated expression of critical viral genes capable of redirecting cellular gene expression. The redirected cellular expression is a consequence of either indirect epigenetic regulation where cellular signaling or transcriptional dysregulation occurs or direct epigenetic regulation where epigenetic cofactors such as histone deacetylases are targeted. In the more complex herpersviruses transformation is a consequence of the expression of the viral latency proteins and RNAs which again can have either a direct or indirect effect on epigenetic regulation of cellular expression. Nevertheless, many questions still remain with respect to the specific mechanisms underlying epigenetic regulation of the viruses and transformation.

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Acknowledgements

This work was supported by National Institute of Health grants AI094441 to B.I.M. and GM098328 to L.B. We thank Christopher Petrides for assistance with Fig. 1.

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Authors and Affiliations

  1. Department of Basic Sciences, School of Medicine and Health Sciences, University of North Dakota, Stop 9037, 501 N. Columbia Rd., Grand Forks, ND, 58202, USA

    Barry I. Milavetz

  2. Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202, USA

    Lata Balakrishnan

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  1. Barry I. Milavetz
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  2. Lata Balakrishnan
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Correspondence to Barry I. Milavetz .

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  1. National Institutes of Health, Rockville, Maryland, USA

    Mukesh Verma

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Milavetz, B.I., Balakrishnan, L. (2015). Viral Epigenetics. In: Verma, M. (eds) Cancer Epigenetics. Methods in Molecular Biology, vol 1238. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1804-1_30

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  • DOI: https://doi.org/10.1007/978-1-4939-1804-1_30

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1803-4

  • Online ISBN: 978-1-4939-1804-1

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