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Study of the Stn Protein in Salmonella; A Regulator of Membrane Composition and Integrity

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Salmonella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1225))

Abstract

Our studies were undertaken to develop new insights into the function of the Salmonella Stn protein. An analysis of total cell membrane protein fraction suggested the possibility that Stn associates with OmpA. This possibility was confirmed by immunogold labeling using anti-OmpA antibody and far-western blotting. From these results, we conclude that Stn regulates membrane composition and integrity in Salmonella.

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Acknowledgement

This work was supported by the Global Center of Excellence Program on Integrated Global Control Strategy for the Tropical and Emerging Infectious Diseases from the Ministry of Education, Culture, Sports, Science and Technology of Japan. JM was supported by the Intramural Research Program, National Institutes of Health, National Heart, Lung, and Blood Institute.

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Authors and Affiliations

  1. Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, 1-12-4, Sakamoto, Nagasaki, 852-8523, Japan

    Masayuki Nakano Ph.D. & Toshiya Hirayama

  2. Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan

    Eiki Yamasaki & Hisao Kurazono

  3. Cardiovascular and Pulmonary Branch, NHLBI, National Institutes of Health, Bethesda, MD, USA

    Joel Moss

Authors
  1. Masayuki Nakano Ph.D.
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  2. Eiki Yamasaki
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  3. Joel Moss
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  4. Toshiya Hirayama
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  5. Hisao Kurazono
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Corresponding author

Correspondence to Masayuki Nakano Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA

    Heide Schatten

  2. Cancer Research Center, University of Missouri, Columbia, Missouri, USA

    Abraham Eisenstark

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© 2015 Springer Science+Business Media New York

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Nakano, M., Yamasaki, E., Moss, J., Hirayama, T., Kurazono, H. (2015). Study of the Stn Protein in Salmonella; A Regulator of Membrane Composition and Integrity. In: Schatten, H., Eisenstark, A. (eds) Salmonella. Methods in Molecular Biology, vol 1225. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1625-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-1625-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1624-5

  • Online ISBN: 978-1-4939-1625-2

  • eBook Packages: Springer Protocols

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