Abstract
During the last decade it has been shown that most mammalian blastocysts consisted of three cell lineages. Immunofluorescence with multiple antibodies enables to identify each cell type allowing an easy detection of eventual defects. It is complementary to RT-PCR experiments as this technique allows to look at cell position and to analyze and count the proportions between the different cell types. Thus after any kind of embryo manipulation such as nuclear transfer (NT), the analysis of the three cell lineages by immunofluorescence will provide criteria for good or poor development.
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Acknowledgement
This work was supported by the ANR “EpiNodal.”
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Chauveau, S., Chazaud, C. (2015). Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining. In: Beaujean, N., Jammes, H., Jouneau, A. (eds) Nuclear Reprogramming. Methods in Molecular Biology, vol 1222. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1594-1_13
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DOI: https://doi.org/10.1007/978-1-4939-1594-1_13
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