Abstract
Advanced high-throughput post-genomic technologies are increasingly being applied in the areas of experimental and clinical medicine. Studies using genomic and transcriptomic approaches currently dominate the arena in every biomedical discipline. However, proteomic techniques are also gaining popularity in the basic and clinical settings. Tissue microarray (TMA) technology is essentially an extension of proteomics, enabling the simultaneous analysis of protein biomarkers in hundreds or thousands of tissue samples in an array format. TMAs are an ordered array of tissue cores on a charged glass slide. They permit immunohistochemical analysis of numerous tissue sections under identical experimental conditions. The arrays can contain samples of every organ in the human body, or a wide variety of common tumors and obscure clinical cases alongside normal controls. The arrays can also contain pellets of cultured cell lines. TMAs may be used like any histological section for immunohistochemistry or even in situ hybridization to detect protein and gene expression. The technology is inexpensive, fast, and statistically powerful. This new technology will allow investigators to analyze numerous biomarkers over essentially identical samples, develop novel prognostic markers, and validate potential drug targets. Therefore, it has substantial potential to facilitate translational research. Despite its impact on cancer research, this important technology has yet to fully penetrate into the area of toxicology. TMA technology is likely to have an increasingly important role in toxicology research. This chapter is intended to provide an introduction to TMA technology, summarize its strengths and weaknesses, and highlight its potential uses in toxicological immunohistochemistry.
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References
Heal JR, Roberts GW, Raynes JG, Bhakoo A, Miller AD, et al. Specific interactions between sense and complementary peptides: the basis for the proteomic code. Chembiochem. 2002;3(2-3):136–51.
McCulloch A, Bassingthwaighte J, Hunter P, et al. Computational biology of the heart: from structure to function. Prog Biophys Mol Biol. 1998;69(2-3):153–5.
Bassingthwaighte JB. Strategies for the physiome project. Ann Biomed Eng. 2000;28(8):1043–58.
Thongboonkerd V. Proteomic analysis of renal diseases: unraveling the pathophysiology and biomarker discovery. Expert Rev Proteomics. 2005;2(3):349–66.
Kennedy S. The role of proteomics in toxicology: identification of biomarkers of toxicity by protein expression analysis. Biomarkers. 2002;7(4):269–90.
Heller MJ. DNA microarray technology: devices, systems, and applications. Annu Rev Biomed Eng. 2002;4:129–53.
Kumble KD. Protein microarrays: new tools for pharmaceutical development. Anal Bioanal Chem. 2003;377(5):812–9.
Sanchez-Carbayo M. Antibody arrays: technical considerations and clinical applications in cancer. Clin Chem. 2006;52(9):1651–9.
Uttamchandani M, Wang J, Yao SQ. Protein and small molecule microarrays: powerful tools for high-throughput proteomics. Mol Biosyst. 2006;2(1):58–68.
Schena M, Shalon D, Davis RW, Brown PO. et al. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science. 1995;270(5235):467–70.
Bubendorf L, Kolmer, M, Kononen, J, et al. Hormone therapy failure in human prostate cancer: analysis by complementary DNA and tissue microarrays. J Natl Cancer Inst. 1999;91(20):1758–64.
Kallioniemi OP. Biochip technologies in cancer research. Ann Med. 2001;33(2):142–7.
Kallioniemi OP, Wagner U, Kononen J, Sauter G, et al. Tissue microarray technology for high-throughput molecular profiling of cancer. Hum Mol Genet. 2001;10(7):657–62.
Henshall S. Tissue microarrays. J Mammary Gland Biol Neoplasia. 2003;8(3):347–58.
Kononen J, Bubendorf L, Kallionimeni A, Bärlund M, et al. Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med. 1998;4(7):844–7.
Wan WH, Fortuna MB, Furmanski P. A rapid and efficient method for testing immunohistochemical reactivity of monoclonal antibodies against multiple tissue samples simultaneously. J Immunol Methods. 1987;103(1):121–9.
Battifora H. The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. Lab Invest. 1986;55(2):244–8.
Mobasheri A, Moskalukb CA, Marplesc D, Shakibaei, M, et al. Expression of aquaporin 1 (AQP1) in human synovitis. Ann Anat. 2010;192(2):116–21.
Floyd RV, Wraya S, MartÃn-Vasallob P, Mobasheri A, et al. Differential cellular expression of FXYD1 (phospholemman) and FXYD2 (gamma subunit of Na, K-ATPase) in normal human tissues: a study using high density human tissue microarrays. Ann Anat. 2010;192(1):7–16.
Mobasheri A, Airley R, Hewitt SM, Marples D, et al. Heterogeneous expression of the aquaporin 1 (AQP1) water channel in tumors of the prostate, breast, ovary, colon and lung: a study using high density multiple human tumor tissue microarrays. Int J Oncol. 2005;26(5):1149–58.
Mobasheri A, Marples D. Expression of the AQP-1 water channel in normal human tissues: a semiquantitative study using tissue microarray technology. Am J Physiol Cell Physiol. 2004;286(3):C529–37.
Mobasheri A, Marples D, Young IS, Floyd RV, et al. Distribution of the AQP4 water channel in normal human tissues: protein and tissue microarrays reveal expression in several new anatomical locations, including the prostate gland and seminal vesicles. Channels (Austin). 2007;1(1):29–38.
Camp RL, Chung GG, Rimm DL. Automated subcellular localization and quantification of protein expression in tissue microarrays. Nat Med. 2002;8(11):1323–7.
Ryan D, Mulrane L, Rexhepaj E, Gallagher WM, et al. Tissue microarrays and digital image analysis, Methods Mol Biol. 2011;691:97–112.
Trujillo E, Gonzalez T, MarÃn R, MartÃn-Vasallo P, Marples D, Mobasheri A. et al. Human articular chondrocytes, synoviocytes and synovial microvessels express aquaporin water channels; upregulation of AQP1 in rheumatoid arthritis. Histol Histopathol. 2004;19(2):435–44.
Acknowledgments
The author wishes to acknowledge the support of Dr. Christopher Moskaluk (University of Virginia), Dr. Stephen Hewitt (TARP Lab, NIH), and Dr. Rachel Airley (University of Huddersfield, UK).
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Mobasheri, A. (2016). Tissue Microarray Technology and Its Potential Applications in Toxicology and Toxicological Immunohistochemistry. In: Aziz, S., Mehta, R. (eds) Technical Aspects of Toxicological Immunohistochemistry. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1516-3_2
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DOI: https://doi.org/10.1007/978-1-4939-1516-3_2
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