Abstract
Angiogenesis, defined as the sprouting of new blood vessels from preexisting ones, is a biological process of great clinical relevance due to its involvement in disease as well as its therapeutic potential for revascularizing ischemic tissues. The embryonic mouse hindbrain provides an excellent model to study the molecular and cellular mechanisms of angiogenesis in vivo due the simple geometry of the hindbrain vasculature and its easy accessibility for fluorescent or histochemical staining, and for image capture and quantitation. This chapter outlines protocols for dissection, staining, and analysis of the mouse embryo hindbrain vasculature.
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Acknowledgements
CR and AF are supported by a Wellcome Trust New Investigator Award to CR [095623/Z11/Z] and AP by a PhD studentship from the British Heart Foundation [FS/10/54/28680].
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Plein, A., Ruhrberg, C., Fantin, A. (2015). The Mouse Hindbrain: An In Vivo Model to Analyze Developmental Angiogenesis. In: Ribatti, D. (eds) Vascular Morphogenesis. Methods in Molecular Biology, vol 1214. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1462-3_3
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DOI: https://doi.org/10.1007/978-1-4939-1462-3_3
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