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Studying Host-Pathogen Interaction Events in Living Mice Visualized in Real Time Using Biophotonic Imaging

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Host-Bacteria Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1197))

Abstract

Despite progress in mouse models of bacterial pathogens, studies are often limited by evaluating infections in an individual organ or tissue or at a given time. Here we present a technique to engineer the pathogen, e.g., Brucella melitensis, with a bioluminescent marker permitting analysis of living bacteria in real time during the infectious process from acute to chronic infection. Using this bioluminescent approach, tissue preference, differences between virulent and mutant bacteria, as well as the response of the bacteria to host metabolites can provide extraordinary data enhancing our understanding of host-pathogen interactions.

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Acknowledgement

This work was supported by NIH grants AI073558 and AI088038 and BARD grant US-4378-11.

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Authors and Affiliations

  1. Department of Pathobiological Sciences, University of Wisconsin-Madison, Office 203, 2015 Linden Dr., Madison, WI, 53706, USA

    Gary Splitter, Jerome Harms & Marina Durward

  2. Department of Medicine, University of Washington, Seattle, WA, 98195, USA

    Erik Petersen

  3. University of Miami, Miami, FL, 33136, USA

    Diogo Magnani

  4. Ohio State University, Wooster, OH, 44691, USA

    Gireesh Rajashekara

  5. National Institute of Animal Biotechnology, University of Hyderabad, Hyderabad, India

    Girish Radhakrishnan

Authors
  1. Gary Splitter
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  2. Jerome Harms
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  3. Erik Petersen
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  4. Diogo Magnani
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  5. Marina Durward
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  6. Gireesh Rajashekara
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  7. Girish Radhakrishnan
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Corresponding author

Correspondence to Gary Splitter .

Editor information

Editors and Affiliations

  1. U1047, UFR Médecine, INSERM, Université Montpellier 1, Nîmes, France

    Annette C. Vergunst

  2. U1047, UFR Médecine, INSERM, Université Montpellier 1, Nîmes, France

    David O'Callaghan

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© 2014 Springer Science+Business Media New York

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Splitter, G. et al. (2014). Studying Host-Pathogen Interaction Events in Living Mice Visualized in Real Time Using Biophotonic Imaging. In: Vergunst, A., O'Callaghan, D. (eds) Host-Bacteria Interactions. Methods in Molecular Biology, vol 1197. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1261-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-1261-2_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1260-5

  • Online ISBN: 978-1-4939-1261-2

  • eBook Packages: Springer Protocols

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