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Functional Analysis of Long Noncoding RNAs in Development and Disease

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Systems Biology of RNA Binding Proteins

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 825))

Abstract

Once viewed as part of the “dark matter” of genome, long noncoding RNAs (lncRNAs), which are mRNA-like but lack open reading frames, have emerged as an integral part of the mammalian transcriptome. Recent work demonstrated that lncRNAs play multiple structural and functional roles, and their analysis has become a new frontier in biomedical research. In this chapter, we provide an overview of different lncRNA families, describe methodologies available to study lncRNA–protein and lncRNA–DNA interactions systematically, and use well-studied lncRNAs as examples to illustrate their functional importance during normal development and in disease states.

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Acknowledgements

We are grateful to Li Yang for critical reading of the manuscript. This work is supported by an AACR-Aflac, Inc., Career Development Award for Pediatric Cancer Research (J.C.Z.) and grants XDA01010206, 2012SSTP01 from CAS and grant 31271376 from NSFC (L.-L. Chen).

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  1. State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Ling-Ling Chen

  2. RNA Biology Program, Sanford Burnham Medical Research Institute, La Jolla, CA, 92037, USA

    Jing Crystal Zhao

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  1. Ling-Ling Chen
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Correspondence to Ling-Ling Chen .

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  1. UCSD Moores Cancer Center, Institute for Genomic Medicine UCSD Stem Cell Program, La Jolla, California, USA

    Gene W. Yeo

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Chen, LL., Zhao, J.C. (2014). Functional Analysis of Long Noncoding RNAs in Development and Disease. In: Yeo, G. (eds) Systems Biology of RNA Binding Proteins. Advances in Experimental Medicine and Biology, vol 825. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1221-6_4

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