Abstract
The lymphocytes of epithelial and lamina proprial compartments of the intestine are phenotypically and functionally distinct and serve a wide range of functions in the intestinal mucosa like regulating intestinal homeostasis, maintaining epithelial barrier function as well as regulating adaptive and innate immune responses. To analyze the role of these cells in different disease states, it is necessary to isolate pure cell populations of the intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) of the gut. In this protocol we describe a method to isolate T cells from IEL and LPL, which can be used for further investigations like comparative studies of mRNA expression, cell proliferation assay, or protein analysis.
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References
Bull DM, Bookman MA (1977) Isolation and functional characterization of human intestinal mucosal lymphoid cells. J Clin Invest 59:966–974
Fiocchi C, Battisto JR, Farmer RG (1979) Gut mucosal lymphocytes in inflammatory bowel disease: isolation and preliminary functional characterization. Dig Dis Sci 24:705–717
Lundqvist C, Hammarstrom ML, Athlin L, Hammarstrom S (1992) Isolation of functionally active intraepithelial lymphocytes and enterocytes from human small and large intestine. J Immunol Methods 152:253–263
Davies MD, Parrott DM (1981) Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine. Gut 22:481–488
Dillon SB, MacDonald TT (1984) Functional properties of lymphocytes isolated from murine small intestinal epithelium. Immunology 52:501–509
Mosley RL, Klein JR (1992) A rapid method for isolating murine intestine intraepithelial lymphocytes with high yield and purity. J Immunol Methods 156:19–26
Lefrancois L, Lycke N (2001) Isolation of mouse small intestinal intraepithelial lymphocytes, Peyer’s patch, and lamina propria cells. In: Coligan JE et al (eds) Current protocols in immunology. Wiley, New York, NY, Chapter 3: Unit 3 19
Weigmann B, Tubbe I, Seidel D, Nicolaev A, Becker C et al (2007) Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue. Nat Protoc 2:2307–2311
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Reißig, S., Hackenbruch, C., Hövelmeyer, N. (2014). Isolation of T Cells from the Gut. In: Waisman, A., Becher, B. (eds) T-Helper Cells. Methods in Molecular Biology, vol 1193. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1212-4_3
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DOI: https://doi.org/10.1007/978-1-4939-1212-4_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1211-7
Online ISBN: 978-1-4939-1212-4
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