Abstract
Some chemicals found in the environment may cause DNA damage. The mutations which may result from this damage can cause various diseases including cancer. It is important to have methods available to test chemicals to which humans may be exposed for their mutagenic potential. In 2011 the Organization for Economic Cooperation and Development adopted a test guideline on the use of transgenic rodent (TGR) assays for investigating the mutagenic potential of chemical agents. The TGRs used in these assays carry a transgene consisting of multiple copies of a bacterial gene which is incorporated into the genome and thus resides in every cell of every tissue. These transgenes have no effect on the animal but are easily recovered and tested for DNA mutations. These TGR assays have an advantage over bacterial and in vitro assays in that the exposure to the test agent occurs within a live animal with all of the various metabolic and DNA repair processes in place, thus more closely modeling actual human exposure. The possibility to investigate tissue specificity by examining DNA from various tissues in the same animal adds to the value of the assay. Herein we describe the use of the Mutaâ„¢Mouse transgenic mouse model for determining the mutagenic potential of chemical agents.
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Gingerich, J.D., Soper, L., Lemieux, C.L., Marchetti, F., Douglas, G.R. (2014). Transgenic Rodent Gene Mutation Assay in Somatic Tissues. In: Sierra, L., GaivĂ£o, I. (eds) Genotoxicity and DNA Repair. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1068-7_18
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DOI: https://doi.org/10.1007/978-1-4939-1068-7_18
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