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A Chip Off the Old Block: The Brain Slice as a Model for Metabolic Studies of Brain Compartmentation and Neuropharmacology

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Brain Energy Metabolism

Part of the book series: Neuromethods ((NM,volume 90))

Abstract

The cortical brain tissue slice is a reductionist model system of the brain, representing interacting, living cells which operate very similarly to intact brain tissue. Metabolic rates are only slightly slower than in heavily anaesthetised brain, and complications introduced by the blood–brain barrier and peripheral metabolism are removed. In this work, we describe how to make and maintain brain tissue slices in biochemical studies, how to use the technique to conduct neurochemical experiments and how to extract metabolic data using NMR spectroscopy. Finally, we describe the use of metabolomics multivariate statistical approaches for obtaining data-driven outcomes in neuropharmacological research.

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Author information

Authors and Affiliations

  1. Neuroscience Research Australia, Barker St, Randwick, NSW, 2031, Australia

    Caroline Rae

  2. The University of New South Wales, Sydney, NSW, 2052, Australia

    Caroline Rae

  3. Discipline of Anatomy and Bosch Institute, School of Medical Sciences, Sydney Medical School, The University of Sydney, Sydney, NSW, 2006, Australia

    Vladimir J. Balcar

Authors
  1. Caroline Rae
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  2. Vladimir J. Balcar
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Corresponding author

Correspondence to Caroline Rae .

Editor information

Editors and Affiliations

  1. Carl-Ludwig-Institute for Physiology, University of Leipzig, Leipzig, Germany

    Johannes Hirrlinger

  2. Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Helle S. Waagepetersen

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Rae, C., Balcar, V.J. (2014). A Chip Off the Old Block: The Brain Slice as a Model for Metabolic Studies of Brain Compartmentation and Neuropharmacology. In: Hirrlinger, J., Waagepetersen, H. (eds) Brain Energy Metabolism. Neuromethods, vol 90. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1059-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1059-5_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1058-8

  • Online ISBN: 978-1-4939-1059-5

  • eBook Packages: Springer Protocols

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