Abstract
In recent years, distinct classes of small RNAs ranging in size from ~21 to 26 nucleotides have been discovered and shown to play important roles in a wide array of cellular functions. Because of the abundance of these small RNAs, library preparation from an RNA sample followed by deep sequencing provides the identity and quantity of a particular class of small RNAs. In this chapter we describe a detailed protocol for preparing small RNA libraries for deep sequencing on the Illumina platform from the nematode C. elegans.
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Ow, M.C., Lau, N.C., Hall, S.E. (2014). Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans . In: Werner, A. (eds) Animal Endo-SiRNAs. Methods in Molecular Biology, vol 1173. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0931-5_6
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DOI: https://doi.org/10.1007/978-1-4939-0931-5_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0930-8
Online ISBN: 978-1-4939-0931-5
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