Abstract
Gene regulation at the level of translation occurs in response to environmental perturbation and is increasingly recognized as a factor affecting plant development. Despite extensive knowledge of transcriptional control, very little is known about translational regulation of genes in response to the daily light/dark cycles. Here we describe the experimental layout designed to address how the translation states of genes change at various times during a diurnal cycle in Arabidopsis thaliana seedlings. We have adopted a strategy combining sucrose-gradient profiling of ribosomes and high-throughput microarray analysis of the ribosome-associated mRNA to investigate the translational landscape of the Arabidopsis genome. This is a powerful technique that can be easily extended to study translation regulation in different genetic backgrounds and under various environmental conditions.
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This work was supported by NSF grant DBI-0820047.
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Missra, A., von Arnim, A.G. (2014). Analysis of mRNA Translation States in Arabidopsis Over the Diurnal Cycle by Polysome Microarray. In: Staiger, D. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 1158. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0700-7_10
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DOI: https://doi.org/10.1007/978-1-4939-0700-7_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0699-4
Online ISBN: 978-1-4939-0700-7
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