Abstract
Temperature Switch PCR (TSP) is a robust single-marker single nucleotide polymorphism (SNP) genotyping technique with broad applications in genetic studies of various organisms. The technique consists of a biphasic PCR with two sets of primers, a locus-specific set and a nested locus-specific set. The PCR products can be easily assessed for polymorphism based on different band sizes using agarose gel electrophoresis.
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Thanh, L.P., Khoo, K. (2014). Temperature Switch PCR (TSP): A Gel-Based Molecular Marker Technique for Investigating Single Nucleotide Polymorphisms. In: Fleury, D., Whitford, R. (eds) Crop Breeding. Methods in Molecular Biology, vol 1145. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0446-4_3
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DOI: https://doi.org/10.1007/978-1-4939-0446-4_3
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