Abstract
Microtubule plus-tip tracking is a powerful method to measure microtubule growth dynamics in vivo. Here we outline an approach that exploits live confocal microscopy of a GFP-tagged EB1-like protein to measure microtubule growth behavior and minus-end-directed microtubule motor activity at the cortex of Caenorhabditis elegans embryos. The EB1 velocity assay (EVA) provides a method to reproducibly monitor motor- and non-motor-assisted microtubule movements.
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Acknowledgements
We thank the Srayko Lab for discussions and comments on this article. This work was supported by the Canadian Institutes of Health Research (CIHR) and Alberta Innovates Technology Futures. M.S. was supported by scholar awards from the Alberta Heritage Foundation for Medical Research and CIHR.
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Tegha-Dunghu, J., Gusnowski, E.M., Srayko, M. (2014). Measuring Microtubule Growth and Gliding in Caenorhabditis elegans Embryos. In: Sharp, D. (eds) Mitosis. Methods in Molecular Biology, vol 1136. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0329-0_7
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DOI: https://doi.org/10.1007/978-1-4939-0329-0_7
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