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Analysis of Global Genome Methylation Using the Cytosine-Extension Assay

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Plant Epigenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1456))

Abstract

DNA methylation is a reversible covalent chemical modification of DNA intended to regulate chromatin structure and gene expression in a cell- and tissue-specific manner and in response to the environment. Cytosine methylation is predominantly occurring in plants, and cytosine nucleotides in plants can be methylated at symmetrical (CpG and CpHpG) and nonsymmetrical sites. Although there exists a number of various methods for the detection of cytosine methylation, most of them are either laborious or expensive or both. Here, we describe a quick inexpensive method for the analysis of global genome methylation using a cytosine-extension assay. The assay can be used for the analysis of the total level of CpG, CpHpG, and CpHpH methylation in a given sample of plant DNA.

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Author information

Authors and Affiliations

  1. Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada

    Andriy Bilichak

  2. Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4

    Igor Kovalchuk

Authors
  1. Andriy Bilichak
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  2. Igor Kovalchuk
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Corresponding author

Correspondence to Andriy Bilichak .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada

    Igor Kovalchuk

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Bilichak, A., Kovalchuk, I. (2017). Analysis of Global Genome Methylation Using the Cytosine-Extension Assay. In: Kovalchuk, I. (eds) Plant Epigenetics. Methods in Molecular Biology, vol 1456. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-4899-7708-3_6

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  • DOI: https://doi.org/10.1007/978-1-4899-7708-3_6

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  • Publisher Name: Humana Press, Boston, MA

  • Print ISBN: 978-1-4899-7706-9

  • Online ISBN: 978-1-4899-7708-3

  • eBook Packages: Springer Protocols

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