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Protein Analysis and Purification

Benchtop Techniques

  • Book
  • © 1996

Overview

Authors:
  1. Ian M. Rosenberg

    1. Massachusetts General Hospital, Boston, USA

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Table of contents (11 chapters)

  1. Front Matter

    Pages i-xxi
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  2. An Overview of this Manual

    • Ian M. Rosenberg
    Pages 1-4

  3. Protein Structure

    • Ian M. Rosenberg
    Pages 5-18

  4. Tracking the Target Protein

    • Ian M. Rosenberg
    Pages 19-54

  5. Electrophoretic Techniques

    • Ian M. Rosenberg
    Pages 55-98

  6. Getting Started with Protein Purification

    • Ian M. Rosenberg
    Pages 99-133

  7. Membrane and Particulate-Associated Proteins

    • Ian M. Rosenberg
    Pages 135-152

  8. Transfer and Detection of Proteins on Membrane Supports

    • Ian M. Rosenberg
    Pages 153-182

  9. Peptide Mapping and Microsequencing

    • Ian M. Rosenberg
    Pages 183-206

  10. Modified Proteins and Peptides

    • Ian M. Rosenberg
    Pages 207-264

  11. Chromatography

    • Ian M. Rosenberg
    Pages 265-324

  12. Recombinant Protein Techniques

    • Ian M. Rosenberg
    Pages 325-363

  13. Back Matter

    Pages 365-434
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Keywords

About this book

This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a biochemical project. The protocols may be performed in the order laid out or may be used inde­ pendently. The aim of the book is to assist a wide range of researchers. from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the promi­ nently numbered steps. you can work your way through any protocol. whether it's a new technique or a task you've done before for which you need a quick review or updated methodology. This manual will assist the experimentalist in designing properly controlled experiments. There will be no advice for dealing with specific pieces of equip­ ment other than encouragement to read the manual, if you can find it. Through­ out all manipulations try to be objective. Be on the lookout for unexpected findings. You will learn the most from unexpected results. and they are often the beginning of the next project. It is never possible to record too much in your lab notebook. Do not get discouraged. Remember, things will not always run smoothly.

Authors and Affiliations

  • Massachusetts General Hospital, Boston, USA

    Ian M. Rosenberg

Bibliographic Information

  • Book Title: Protein Analysis and Purification

  • Book Subtitle: Benchtop Techniques

  • Authors: Ian M. Rosenberg

  • DOI: https://doi.org/10.1007/978-1-4757-1108-0

  • Publisher: Birkhäuser Boston, MA

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1996

  • eBook ISBN: 978-1-4757-1108-0Published: 14 March 2013

  • Edition Number: 1

  • Number of Pages: XXI, 434

  • Number of Illustrations: 76 b/w illustrations

  • Topics: Mathematics, general

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