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Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation

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Bone Marrow and Stem Cell Transplantation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1109))

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Abstract

Infection remains an important source of morbidity and mortality in patients who undergo hematopoietic stem cell transplantation (HSCT). In the immune reconstitution period after transplantation, HSCT recipients are most likely to have bacterial or fungal infections. Invasive fungal infections (IFIs) and mycobacterial infections (MBIs) are among the complications of HSCT, with high morbidity and mortality rates. Early diagnosis of both is crucial in order to manipulate the disease and to avoid fulminant outcomes. This chapter reviews the current knowledge on the molecular diagnosis of IFIs and MBIs in HSCT recipients, describing two different polymerase chain reaction (PCR)-based methods, one commercial (qPCR, Roche) and one in-house IS6110-based protocol.

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Notes

  1. 1.

    M. tuberculosis, M. africanum, M. microti, M. canetti, and M. bovis including bacillus Calmette–Guerin (BCG) group.

  2. 2.

    Most commonly encountered species in HSCT: M. fortuitum, M. chelonae, M. abscessus, and M. avium complex; for more detailed information on NTM, see ref. 6.

  3. 3.

    Predominantly M. fortuitum, M. abscessus, and M. chelonae.

  4. 4.

    M. tuberculosis strains which are resistant to at least rifampicin and isoniazid.

  5. 5.

    M. tuberculosis strains which are resistant to any fluoroquinolone and one of the three injectable aminoglycosides (capreomycin, kanamycin, and amikacin) in addition to rifampicin and isoniazid.

  6. 6.

    POC tests are diagnostic tests that do not require laboratory facilities or specialist training and that can be used where care is provided within a clinic or in the community.

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Authors and Affiliations

  1. Ankara University Faculty of Medicine, Cord Blood Bank, Ankara, Turkey

    Pinar Yurdakul

  2. Department of Microbiology and Clinical Microbiology, Baskent University Faculty of Medicine Adana Hospital, Adana, Turkey

    Sule Colakoglu

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  1. Pinar Yurdakul
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  2. Sule Colakoglu
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Editors and Affiliations

  1. Department of Hematology, Ankara University, Ankara, Turkey

    Meral Beksaç

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Yurdakul, P., Colakoglu, S. (2014). Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation. In: Beksaç, M. (eds) Bone Marrow and Stem Cell Transplantation. Methods in Molecular Biology, vol 1109. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9437-9_13

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  • DOI: https://doi.org/10.1007/978-1-4614-9437-9_13

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  • Print ISBN: 978-1-4614-9436-2

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