Abstract
Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize the widely used protocols for pollen viability staining; the investigation of anther morphogenesis by light microscopy of semi-thin sections; TUNEL assay for programmed tapetum cell death; and laser microdissection procedures to obtain specialized cells or cell layers for carrying out transcriptomics.
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Chang, F., Zhang, Z., Jin, Y., Ma, H. (2014). Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice. In: Riechmann, J., Wellmer, F. (eds) Flower Development. Methods in Molecular Biology, vol 1110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9408-9_9
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DOI: https://doi.org/10.1007/978-1-4614-9408-9_9
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