Abstract
Large-scale profiling techniques have been increasingly applied to the analysis of genetically engineered (GE) crop plants with regard to their food safety and nutritional equivalence. Although metabolomics are becoming the prevalent approach, proteomics are also used to detect unintended effects that may be triggered by insertion of a transgene. In this chapter we review 16 articles that used two-dimensional electrophoresis and (in most cases) peptide mass spectrometry as analytical methods for GE crops (grapevine, maize, pea, potato, rice, soybean, tomato, and wheat). Some relevant articles studying the laboratory model plant Arabidopsis thaliana are also summarized. These articles converge to show minimal unintended effects due to the transformation events. The transgenic genetic modification itself has less impact on protein content than conventional plant breeding or the environment. None of these papers has raised new safety concerns about marketed GE varieties. We also provide examples of two-dimensional electrophoresis protein analysis as an approach for detecting potential allergens in GE crop-derived food.
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Ricroch, A.E., Kuntz, M. (2013). Evaluation of Genetically Engineered Crops Using Proteomics. In: Toldrá, F., Nollet, L. (eds) Proteomics in Foods. Food Microbiology and Food Safety, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-5626-1_25
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DOI: https://doi.org/10.1007/978-1-4614-5626-1_25
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