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Doppler Optical Coherence Tomography

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Abstract

This chapter describes Doppler optical coherence tomography (D-OCT). This is an imaging modality that combines Doppler principles with optical coherence tomography to image tissue structure and blood flow velocity simultaneously. We will review the principle and technology of D-OCT and illustrate a few examples of its applications.

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References

  1. P.L. Carson, D.D. Adler, J.B. Fowlkes, Enhanced color flow imaging of breast cancer vasculature: continuous wave Doppler and three-dimensional display. J. Ultrasound Med. 11, 77 (1992)

    Google Scholar 

  2. D. Huang, E.A. Swanson, C.P. Lin, J.S. Schuman, W.G. Stinson, W. Chang, M.R. Hee, T. Flotte, K. Gregory, C.A. Puliafito, J.G. Fujimoto, Optical coherence tomography. Science 254, 1178–1181 (1991)

    Article  ADS  Google Scholar 

  3. V. Gusmeroli, M. Martnelli, Distributed laser Doppler velocimeter. Opt. Lett. 16, 1358–1360 (1991)

    Article  ADS  Google Scholar 

  4. Z. Chen, T.E. Milner, S. Srinivas, X.J. Wang, A. Malekafzali, M.J.C. van Gemert, J.S. Nelson, Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography. Opt. Lett. 22, 1119–1121 (1997)

    Article  ADS  Google Scholar 

  5. J.A. Izatt, M.D. Kulkarni, S. Yazdanfar, J.K. Barton, A.J. Welch, In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography. Opt. Lett. 22, 1439–1441 (1997)

    Article  ADS  Google Scholar 

  6. Z. Chen, T.E. Milner, D. Dave, J.S. Nelson, Optical Doppler tomographic imaging of fluid flow velocity in highly scattering media. Opt. Lett. 22, 64–66 (1997)

    Article  ADS  Google Scholar 

  7. Z. Chen, T.E. Milner, X.J. Wang, S. Srinivas, J.S. Nelson, Optical Doppler tomography: imaging in vivo blood flow dynamics following pharmacological intervention and photodynamic therapy. Photochem. Photobiol. 67, 56–60 (1998)

    Article  Google Scholar 

  8. Z. Chen, Y. Zhao, S.M. Srinivas, J.S. Nelson, N. Prakash, R.D. Frostig, Optical Doppler tomography. IEEE J. Sel. Top. Quant. Electron. 5, 1134–1141 (1999)

    Article  Google Scholar 

  9. M.D. Kulkarni, T.G. van Leeuwen, S. Yazdanfar, J.A. Izatt, Velocity-estimation accuracy and frame-rate limitations in color Doppler optical coherence tomography. Opt. Lett. 23, 1057–1059 (1998)

    Article  ADS  Google Scholar 

  10. Y. Zhao, Z. Chen, C. Saxer, S. Xiang, J.F. de Boer, J.S. Nelson, Phase-resolved optical coherence tomography and optical Doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity. Opt. Lett. 25, 114–116 (2000)

    Article  ADS  Google Scholar 

  11. Y. Zhao, Z. Chen, C. Saxer, Q. Shen, S. Xiang, J.F. de Boer, J.S. Nelson, Doppler standard deviation imaging for clinical monitoring of in vivo human skin blood flow. Opt. Lett. 25, 1358–1360 (2000)

    Article  ADS  Google Scholar 

  12. Y. Zhao, Z. Chen, Z. Ding, H. Ren, J.S. Nelson, Three-dimensional reconstruction of in vivo blood vessels in human skin using phase-resolved optical Doppler tomography. IEEE J. Sel. Top. Quant. Electron. 7, 931–935 (2001)

    Article  Google Scholar 

  13. Z. Ding, Y. Zhao, H. Ren, S.J. Nelson, Z. Chen, Real-time phase resolved optical coherence tomography and optical Doppler tomography. Opt. Express 10, 236–245 (2002)

    Article  ADS  Google Scholar 

  14. J.S. Nelson, K.M. Kelly, Y. Zhao, Z. Chen, Imaging blood flow in human port-wine stain in situ and in real time using optical Doppler tomography. Arch. Dermatol. 137, 741–744 (2001)

    Google Scholar 

  15. V.X. Yang, M.L. Gordon, A. Mok, Y. Zhao, Z. Chen, R.S.C. Cobbold, B.C. Wilson, I.A. Vitkin, Improved phase-resolved optical Doppler tomography using the Kasai velocity estimator and histogram segmentation. Opt. Commun. 208, 209–214 (2002)

    Article  ADS  Google Scholar 

  16. V. Westphal, S. Yazdanfar, A.M. Rollins, J.A. Izatt, Real-time, high velocity-resolution color Doppler optical coherence tomography. Opt. Lett. 27, 34–36 (2002)

    Article  ADS  Google Scholar 

  17. D.P. Dave, T.E. Milner, Doppler-angle measurement in highly scattering media. Opt. Lett. 25, 1523–1525 (2000)

    Article  ADS  Google Scholar 

  18. H. Ren, M.K. Breke, Z. Ding, Y. Zhao, J.S. Nelson, Z. Chen, Imaging and quantifying transverse flow velocity with the Doppler bandwidth in a phase-resolved functional optical coherence tomography. Opt. Lett. 27, 409–411 (2002)

    Article  ADS  Google Scholar 

  19. L. Yu, Z. Chen, Doppler variance imaging for three-dimensional retina and choroid angiography. J. Biomed. Opt. 15, 016029 (2010)

    Article  MathSciNet  ADS  Google Scholar 

  20. L. Yu, E. Nguyen, G. Liu, B. Choi, Z. Chen, Spectral Doppler optical coherence tomography imaging of localized ischemic stroke in a mouse model. J. Biomed. Opt. 15, 066006 (2010)

    Article  ADS  Google Scholar 

  21. G. Liu, M. Rubinstein, A. Saidi, W. Qi, A. Foulad, B. Wong, Z. Chen, Imaging vibrating vocal folds with a high speed 1050 nm swept source OCT and ODT. Opt. Express 19, 11880–11889 (2011)

    Article  Google Scholar 

  22. G. Liu, W. Qi, L. Yu, Z. Chen, Real-time bulk-motion-correction free Doppler variance optical coherence tomography for choroidal capillary vasculature imaging. Opt. Express 19, 3657–3666 (2011)

    Article  ADS  Google Scholar 

  23. S. Makita, Y. Hong, M. Yamanari, T. Yatagai, Y. Yasuno, Optical coherence angiography. Opt. Express 14, 7821–7840 (2006)

    Article  ADS  Google Scholar 

  24. Y. Hong, S. Makita, M. Yamanari, M. Miura, S. Kim, T. Yatagai, Y. Yasuno, Three-dimensional visualization of choroidal vessels by using standard and ultra-high resolution scattering optical coherence angiography. Opt. Express 15, 7538–7550 (2007)

    Article  ADS  Google Scholar 

  25. Y. Yasuno, Y. Hong, S. Makita, M. Yamanari, M. Akiba, M. Miura, T. Yatagai, In vivo high-contrast imaging of deep posterior eye by 1-micron swept source optical coherence tomography and scattering optical coherence angiography. Opt. Express 15, 6121–6139 (2007)

    Article  ADS  Google Scholar 

  26. L. An, R.K. Wang, In vivo volumetric imaging of vascular perfusion within human retina and choroids with optical micro-angiography. Opt. Express 16, 11438–11452 (2008)

    Article  ADS  Google Scholar 

  27. R.K. Wang, L. An, P. Francis, D.J. Wilson, Depth-resolved imaging of capillary networks in retina and choroid using ultrahigh sensitive optical microangiography. Opt. Lett. 35, 1467–1469 (2010)

    Article  ADS  Google Scholar 

  28. R.K. Wang, L. An, S. Saunders, D.J. Wilson, Optical microangiography provides depth-resolved images of directional ocular blood perfusion in posterior eye segment. J. Biomed. Opt. 15, 020502 (2010)

    Article  ADS  Google Scholar 

  29. M. Szkulmowski, I. Grulkowski, D. Szlag, A. Szkulmowska, A. Kowalczyk, M. Wojtkowski, Flow velocity estimation by complex ambiguity free joint spectral and time domain optical coherence tomography. Opt. Express 17, 14281–14297 (2009)

    Article  ADS  Google Scholar 

  30. I. Grulkowski, I. Gorczynska, M. Szkulmowski, D. Szlag, A. Szkulmowska, R.A. Leitgeb, A. Kowalczyk, M. Wojtkowski, Scanning protocols dedicated to smart velocity ranging in spectral OCT. Opt. Express 17, 23736–23754 (2009)

    Article  Google Scholar 

  31. J. Barton, S. Areomaki, Flow measurement without phase information in optical coherence tomography. Opt. Express 13, 5483–5493 (2005)

    Article  Google Scholar 

  32. A. Mariampillai, B.A. Standish, E.H. Moriyama, M. Khurana, N.R. Munce, M.K. Leung, J. Jiang, A. Cable, B.C. Wilson, I.A. Vitkin, V.X. Yang, Speckle variance detection of microvasculature using swept-source optical coherence tomography. Opt. Lett. 33, 1530–1532 (2008)

    Article  ADS  Google Scholar 

  33. A. Mariampillai, M.K. Leung, M. Jarvi, B.A. Standish, K. Lee, B.C. Wilson, A. Vitkin, V.X. Yang, Optimized speckle variance OCT imaging of microvasculature. Opt. Lett. 35, 1257–1259 (2010)

    Article  ADS  Google Scholar 

  34. E. Jonathan, J. Enfield, M.J. Leahy, Correlation mapping method for generating microcirculation morphology from optical coherence tomography (OCT) intensity images. J. Biophotonics 4, 583–587 (2010)

    Google Scholar 

  35. G. Liu, L. Chou, W. Jia, W. Qi, B. Choi, Z. Chen, Intensity-based modified Doppler variance algorithm: application to phase instable and phase stable optical coherence tomography systems. Opt. Express 19, 11429–11440 (2011)

    Article  ADS  Google Scholar 

  36. Y. Jia, M.R. Grafe, A. Gruber, N.J. Alkayed, R.K. Wang, In vivo optical imaging of revascularization after brain trauma in mice. Microvasc. Res. 81, 73–80 (2010)

    Article  Google Scholar 

  37. Y. Jia, R.K. Wang, Optical micro-angiography images structural and functional cerebral blood perfusion in mice with cranium left intact. J. Biophotonics 4, 57–63 (2011)

    Article  Google Scholar 

  38. V.J. Srinivasan, S. Sakadzic, I. Gorczynska, S. Ruvinskaya, W. Wu, J.G. Fujimoto, D.A. Boas, Quantitative cerebral blood flow with optical coherence tomography. Opt. Express 18, 2477–2494 (2010)

    Article  Google Scholar 

  39. B.J. Vakoc, R.M. Lanning, J.A. Tyrrell, T.P. Padera, L.A. Bartlett, T. Stylianopoulos, L.L. Munn, G.J. Tearney, D. Fukumura, R.K. Jain, B.E. Bouma, Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging. Nat. Med. 15, 1219–1223 (2009)

    Article  Google Scholar 

  40. S. Yazdanfar, A.M. Rollins, J.A. Izatt, Imaging and velocimetry of the human retinal circulation with color Doppler optical coherence tomography. Opt. Lett. 25, 1448–1450 (2000)

    Article  ADS  Google Scholar 

  41. R.A. Leitgeb, L. Schmetterer, W. Drexler, A.F. Fercher, R.J. Zawadzki, T. Bajraszewski, Real-time assessment of retinal blood flow with ultrafast acquisition by color Doppler Fourier domain optical coherence tomography. Opt. Express 11, 3116–3121 (2003)

    Article  ADS  Google Scholar 

  42. B.R. White, M.C. Pierce, N. Nassif, B. Cense, B.H. Park, G.J. Tearney, B.E. Bouma, T.C. Chen, J.F. de Boer, In vivo dynamic human retinal blood flow imaging using ultra-high speed spectral domain optical Doppler tomography. Opt. Express 25, 3490–3497 (2003)

    Article  ADS  Google Scholar 

  43. Y. Wang, A. Fawzi, O. Tan, J. Gil-Flamer, D. Huang, Retinal blood flow detection in diabetic patients by Doppler Fourier domain optical coherence tomography. Opt. Express 17, 4061–4073 (2009)

    Article  ADS  Google Scholar 

  44. Y. Wang, B.A. Bower, J.A. Izatt, O. Tan, D. Huang, In vivo total retinal blood flow measurement by Fourier domain Doppler optical coherence tomography. J. Biomed. Opt. 12, 041215 (2007)

    Article  ADS  Google Scholar 

  45. B. Rao, L. Yu, H.K. Jiang, L.C. Zacharias, R.M. Kurtz, B.D. Kuppermann, Z. Chen, Imaging pulsatile retinal blood flow in human eye. J. Biomed. Opt. 5, 040505 (2008)

    Article  Google Scholar 

  46. R.K. Wang, L. An, Doppler optical micro-angiography for volumetric imaging of vascular perfusion in vivo. Opt. Express 17, 8926–8940 (2009)

    Article  ADS  Google Scholar 

  47. V.X. Yang, M.L. Gordon, S. Tang, N.E. Marcon, G. Gardiner, B. Qi, S. Bisland, E. Seng-Yue, S. Lo, J. Pekar, B.C. Wilson, I.A. Vitkin, High speed, wide velocity dynamic range Doppler optical coherence tomography (part III): in vivo endoscopic imaging of blood flow in the rat and human gastrointestinal tracts. Opt. Express 11, 2416–2424 (2003)

    Article  ADS  Google Scholar 

  48. V.X. Yang, S.J. Tang, M.L. Gordon, B. Qi, G. Gardiner, M. Cirocco, P. Kortan, G.B. Haber, G. Kandel, I.A. Vitkin, B.C. Wilson, N.E. Marcon, Endoscopic Doppler optical coherence tomography in the human GI tract: initial experience. Gastrointest. Endosc. 61, 879–890 (2006)

    Article  Google Scholar 

  49. D.Y. Kim, J. Fingler, J.S. Werner, D.M. Schwartz, S.E. Fraser, R.J. Zawadzki, In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography. Biomed. Opt. Express 2, 1504–1513 (2011)

    Article  Google Scholar 

  50. G.J. Tearney, B.E. Bouma, J.G. Fujimoto, High-speed phase- and group-delay scanning with a grating-based phase control delay line. Opt. Lett. 22, 1811–1813 (1997)

    Article  ADS  Google Scholar 

  51. F. Hlawatsch, G.F. Boudreaux-Bartels, Linear and quadratic time-frequency signal representations. IEEE Spectr. 4, 21–67 (1992)

    Google Scholar 

  52. S. Yazdanfar, A.M. Rollins, J.A. Izatt, Ultrahigh velocity resolution imaging of the microcirculation in vivo using colar Doppler optical coherence tomography. Proc. SPIE 4251, 156 (2001)

    Article  ADS  Google Scholar 

  53. A.F. Fercher, C.K. Kitzenberger, G. Kamp, S.Y. El-Zaiat, Measurement of intraocular distances by backscattering spectral interferometry. Opt. Commun. 117, 43–48 (1995)

    Article  ADS  Google Scholar 

  54. R. Leitgeb, C.K. Hitzenberger, A.F. Fercher, M. Kulhavy, Performance of Fourier domain vs. time domain optical coherence tomography. Opt. Express 11, 889–894 (2003)

    Article  ADS  Google Scholar 

  55. M.A. Choma, M.V. Sarunic, C. Yang, J.A. Izatt, Sensitivity advantage of swept source and Fourier domain optical coherence tomography. Opt. Express 11, 2183–2189 (2003)

    Article  ADS  Google Scholar 

  56. J.F. de Boer, B. Cense, B.H. Park, M.C. Pierce, G.J. Tearney, B.E. Bouma, Improved signal-to-noise ratio in spectral-domain compared with time-domain optical coherence tomography. Opt. Lett. 28, 2067–2069 (2003)

    Article  ADS  Google Scholar 

  57. B.E. Bouma, G.J. Tearney, Handbook of Optical Coherence Tomography (Marcel Dekker, New York, 2002)

    Google Scholar 

  58. M. Wojtkowski, V.J. Srinivasan, T. Ko, J.G. Fujimoto, A. Kowalczyk, J.S. Duker, Ultrahigh-resolution high speed Fourier domain optical coherence tomography and methods for dispersion compensation. Opt. Express 12, 2404–2422 (2004)

    Article  ADS  Google Scholar 

  59. B. Cense, N. Nassif, T.C. Chen, M.C. Pierce, S.H. Yun, B.H. Park, B.E. Bouma, G.J. Tearney, J.F. de Boer, Ultrahigh-resolution high-speed retinal imaging using spectral-domain optical coherence tomography. Opt. Lett. 12, 2435–2447 (2004)

    Google Scholar 

  60. S.H. Yun, G.J. Tearney, J.F. de Boer, N. Iftimia, B.E. Bouma, High speed optical frequency domain imaging. Opt. Express 11, 2593–2963 (2003)

    Google Scholar 

  61. S.H. Yun, C. Boudoux, G.J. Tearney, B.E. Bouma, High-speed wavelength-swept semiconductor laser with a polygon-scanner-based wavelength filter. Opt. Lett. 28, 1981–1983 (2003)

    Article  ADS  Google Scholar 

  62. J. Zhang, J.S. Nelson, Z. Chen, Removal of mirror image and enhancement of signal to noise ratio in Fourier domain optical coherence tomography using an electro-optical phase modulator. Opt. Lett. 30, 147–149 (2005)

    Article  ADS  Google Scholar 

  63. J. Zhang, J.S. Nelson, Z. Chen, Full range polarization-sensitive Fourier domain optical coherence tomography. Opt. Express 12, 6033–6039 (2004)

    Article  ADS  Google Scholar 

  64. M.V. Sarunic, M.A. Choma, C. Yang, J.A. Izatt, Instantaneous complex conjugate resolved spectral domain and swept-source OCT using 3×3 fiber couplers. Opt. Express 13, 957–967 (2005)

    Article  ADS  Google Scholar 

  65. Z. Chen, in Optical Doppler Tomography for High Resolution Imaging of In Vivo Microcirculation. Whitake Foundation, 1997

    Google Scholar 

  66. R. Leitgeb, L. Schmetterer, M. Wojtkowski, M. Sticker, C.K. Hitzenberger, A.F. Fercher, Flow velocity measurement by frequency domain short coherence interferometry. Proc. SPIE 4619, 16 (2002)

    Article  ADS  Google Scholar 

  67. L. Wang, X. Wei, Y. Wang, M. Bachaman, G.P. Li, Z. Chen, Imaging and quantifying of microflow by phase-resolved optical Doppler tomography. Opt. Commun. 232, 25–29 (2004)

    Article  ADS  Google Scholar 

  68. D. Piao, L.L. Otis, Q. Zhu, Doppler angle and flow velocity mapping by combine Doppler shift and Doppler bandwidth measurements in optical Doppler tomography. Opt. Lett. 28, 1120 (2003)

    Article  ADS  Google Scholar 

  69. S. Proskurin, Y. He, R. Wang, Determination of flow velocity vector based on Doppler shift and spectrum broadening with optical coherence tomography. Opt. Lett. 28, 1227 (2003)

    Article  ADS  Google Scholar 

  70. L. Wang, Development of Phase-Resolved Optical Doppler Tomography for Imaging and Quantifying Microflow Dynamics and Particle Size in Microfluidic Channels (Department of Electrical and Computer Engineering, University of California, Irvine, Irvine, 2004)

    Google Scholar 

  71. Y.-C. Ahn, W. Jung, Z. Chen, Quantification of a three-dimensional velocity vector using spectral-domain Doppler optical coherence tomography. Opt. Lett. 32, 1587–1589 (2007)

    Article  ADS  Google Scholar 

  72. A. Major, S. Kimel, S. Mee, T.E. Milner, D.J. Smithies, S.M. Srinivas, Z. Chen, J.S. Nelson, Microvascular photodynamic effects determined in vivo using optical Doppler tomography. IEEE J. Sel. Top. Quant. Electron. 5, 1168–1175 (1999)

    Article  Google Scholar 

  73. R.D. Frostig, E.E. Lieke, D.Y. Ts'o, A. Grinvald, Cortical functional architechture and local coupling between neuronal activity and the microcirculation revealed by in vivo high-resolution optical imaging of intrinsic signals. Proc. Natl. Acad. Sci. USA 87, 6082–6086 (1990)

    Article  ADS  Google Scholar 

  74. Y. Chen, Z. Chen, Y. Zhao, J.S. Nelson, M. Bachman, Y. Chiang, C. Chu, G.P. Li, Test channels for flow characterization of processed platic microchannels, in 1999 Fall MRS Conference (MRS, Boston, 1999)

    Google Scholar 

  75. Y. Chen, In vivo measurement and characterization of fluid flow in microchannels using OCT/ODT system, in Electrical Engineering (University of California, Irvine, Irvine, 2001), p. 100

    Google Scholar 

  76. Y.-C. Ahn, W. Jung, J. Zhang, Z. Chen, Investigation of laminar dispersion with optical coherence tomography and optical Doppler tomography. Opt. Express 13, 8164–8171 (2005)

    Article  ADS  Google Scholar 

  77. Y.-C. Ahn, W. Jung, Z. Chen, Optical sectioning for microfluidics: secondary flow and mixing in a meandering microchannel. Lab on a Chip 8, 125–133 (2008)

    Article  Google Scholar 

  78. L. Wang, Y. Wang, M. Bachaman, G.P. Li, Z. Chen, Frequency domain Phase-resolved optical Doppler and Doppler variance tomography. Opt. Comm. 242, 345–347 (2004)

    Article  ADS  Google Scholar 

  79. L. Wang, W. Xu, M. Bachaman, G.P. Li, Z. Chen, Phase-resolved optical Doppler tomography for imaging flow dynamics in microfluidic channels. Appl. Phys. Lett. 85, 1855–1857 (2004)

    Article  ADS  Google Scholar 

  80. R.K. Wang, High-resolution visualization of fluid dynamics with Doppler optical coherence tomography. Meas. Sci. Technol. 15, 725–733 (2004)

    Article  ADS  Google Scholar 

  81. Y.-C. Ahn, W. Jung, Z. Chen, Tubid two-phase slog flow in a microtube simultaneous visualizaion of structure and velocity field. Appl. Phys. Lett. 89, 064109 (2006)

    Article  ADS  Google Scholar 

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Acknowledgments

We would like to thank many of our colleagues who have contributed to the functional OCT project at the Beckman Laser Institute and the Department of Biomedical Engineering at UCI, particularly the students and postdoctoral fellows whose hard work made it possible for us to review many of the exciting results in this chapter. Dr. Chen would like to acknowledge the research grants awarded from the National Institutes of Health (R01EB-00293, R01CA-91717, R01EB-10090, R01HL-105215, R01EY-021529, and P41EB-015890), the National Science Foundation (BES-86924), the Whitaker Foundation (WF-23281), and the Defense Advanced Research Program Agency (Bioflip program). Institutional support from the Air Force Office of Scientific Research (FA9550-04-0101) and the Beckman Laser Institute Endowment are also gratefully acknowledged. Dr. Chen’s e-mail address is z2chen@uci.edu.

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Chen, Z., Liu, G. (2013). Doppler Optical Coherence Tomography. In: Tuchin, V. (eds) Handbook of Coherent-Domain Optical Methods. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-5176-1_20

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