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Microdialysis in Metabolic Research

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Microdialysis in Drug Development

Part of the book series: AAPS Advances in the Pharmaceutical Sciences Series ((AAPS,volume 4))

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Abstract

Clinical metabolic research needs tools to get a footprint of human physiology and pathophysiology in vivo. The novel idea of monitoring interstitial fluid by microdialysis in the brain of laboratory animals was a complement to ordinary blood sampling. Semipermeable hollow fibers were implemented in the brain-tissue mimicking characteristics of artificial blood vessels communicating with freely diffusing molecules in situ. Subcutaneous microdialysis for measurements of interstitial concentrations of any low molecular compound was introduced in 1987. Each microdialysis catheter required calibration in situ by the “no net flux” method to get the “true” interstitial glucose, lactate, adenosine, and glycerol concentrations. Addition of 133Xe-clearance assessments of adipose tissue blood flow (ATBF) allowed adoption of Fick’s principle to get an estimate of substrate release. By the time, technical improvements of probes and recovery techniques have made it possible to monitor interstitial insulin and cytokines/chemokines in both sc adipose tissue and muscle. In fact, almost every organ in the human body may be accessible for monitoring of small and large molecules in the interstitial fluid. Thus, the future for the microdialysis technique in metabolic research looks bright.

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Acknowledgments

In the mid-1980s, I was fortunate to start my research career in Ulf Smiths laboratory when Peter Lönnroth decided to test the feasibility of microdialysis, a well-known procedure in neurobiology, for monitoring of adipose tissue metabolism in humans. I am grateful to both of you for the fact that we, more or less, have managed to walk the long and winding road together. Also thank you to all research friends at the University of Gothenburg and all over the world for nice microdialysis collaborations. Finally, I would like to thank my research group, in particular Lena Strindberg whose expertise in running clinical microdialysis experiments is unique.

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  1. The Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy at University of Gothenburg, Sahlgrenska University Hospital, Bruna Stråket 16, Level 2/3, S-413 45, Gothenburg, Sweden

    Per-Anders Jansson

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Correspondence to Per-Anders Jansson .

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  1. Allgemeines Krankenhaus, Department of Clinical Pharmacology, Medical University Vienna, Währinger Gürtel 18-20, Vienna, 1090, Austria

    Markus Müller

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Jansson, PA. (2013). Microdialysis in Metabolic Research. In: Müller, M. (eds) Microdialysis in Drug Development. AAPS Advances in the Pharmaceutical Sciences Series, vol 4. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-4815-0_12

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