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Panfungal PCR Method for Detection of Aflatoxigenic Molds

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Laboratory Protocols in Fungal Biology

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

Aflatoxigenic molds contaminate the processed or raw agricultural ­commodities during their storage and transportation. Traditional methods offer a laborious and tedious process; also, detection can be performed only after toxin production. Molecular methods provide a rapid, sensitive, and specific detection. The major advantage of molecular methods is that the detection can be observed prior to the toxin production. Molecular methods for fungal detection can be applied to distinguish the aflatoxin-secreting fungi from non-aflatoxigenic molds. The DNA-based PCR amplification of a consensus sequence can make it possible to monitor the range of aflatoxigenic fungi from contaminated products.

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References

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Acknowledgements

M. M. Ahmad is thankful to receive RFSMS grant-aided support from UGC, Government of India, India.

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Authors and Affiliations

  1. Department of Biotechnology, Hamdard University, New Delhi, 110062, India

    Malik M. Ahmad, Pravej Alam & M. Z. Abdin

  2. Department of Biochemistry, Hamdard University, New Delhi, 110062, India

    Saleem Javed

Authors
  1. Malik M. Ahmad
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  2. Pravej Alam
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  3. M. Z. Abdin
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  4. Saleem Javed
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Corresponding author

Correspondence to M. Z. Abdin .

Editor information

Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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Ahmad, M.M., Alam, P., Abdin, M.Z., Javed, S. (2013). Panfungal PCR Method for Detection of Aflatoxigenic Molds. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_47

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