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Large-Scale Production of Lignocellulolytic Enzymes in Thermophilic Fungi

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Laboratory Protocols in Fungal Biology

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

A semi-pilot scale process for the production of lignocellulolytic enzymes in the thermophilic fungus Talaromyces emersonii is presented. Each step involved in the scale-up process is described in detail.

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References

  1. Stolk AC, Sampson RA (1972) The genus Talaromyces: studies in mycology, 2nd edn. Centraal bureau Voor Schimmelcultures Publishers, Baarn, The Netherlands

    Google Scholar 

  2. Tuohy MG, Murray PG, Gilleran CT, Collins CM, Reen FJ, McLoughlin L et al. (2007). Talaromyces emersonii enzyme systems. Patent WO/2007/091231.

    Google Scholar 

  3. Polizeli ML, Rizzatti AC, Monti R, Terenzi HF, Jorge JA, Amorim DS (2005) Xylanases from fungi: properties and industrial applications. Appl Microbiol Biotechnol 67:577–591

    Article  PubMed  CAS  Google Scholar 

  4. Murray PG, Collins C, Grassick A, Penttila M, Saloheimo M, Tuohy MG (2004) Expression in Trichoderma ­reesei and characterisation of a thermostable family 3 β-glucosidase from the moderately thermophilic fungus Talaromyces emersonii. Protein Expr Purif 38:248–257

    Article  PubMed  CAS  Google Scholar 

  5. Tuohy MG, Puls J, Claeyssens M, Vrsanska M, Coughlan MP (1993) The xylan-degrading enzyme system of Talaromyces emersonii: novel enzymes with activity against aryl β-D-xylosides and unsubstituted xylans. Biochem J 290:515–523

    PubMed  CAS  Google Scholar 

  6. McCarthy T, Hanniffy O, Savage AV, Tuohy MG (2003) Catalytic properties and mode of action of three endo-betaglucanases from Talaromyces emersonii on soluble b-1,4- and b-1,3;1,4-linked glucans. Int J Biol Macromol 33:141–148

    Article  PubMed  CAS  Google Scholar 

  7. Waters DM, Murray PG, Ryan LA, Arendt EK, Tuohy MG (2010) Talaromyces emersonii thermostable enzyme systems and their applications in wheat baking systems. J Agric Food Chem 58:7415–7422

    Article  PubMed  CAS  Google Scholar 

  8. Gilleran CT, Hernon AT, Murray PG, Tuohy MG (2010) Induction of enzyme cocktails by low cost carbon sources for production of monosaccharide-rich syrups from plant materials. BioResources 5:634–649

    CAS  Google Scholar 

  9. McCarthy T, Hanniffy O, Lalor E, Savage AV, Tuohy MG (2005) Evaluation of three thermostable fungal endo-β-glucanases from Talaromyces emersonii for brewing and food applications. Proc Biochem 40:1741–1748

    Article  CAS  Google Scholar 

  10. Waters DM, Ryan LA, Murray PG, Arendt EK, Tuohy MG (2011) Characterization of a Talaromyces emersonii thermostable enzyme cocktail with applications in wheat dough rheology. Enzyme Microb Technol 49:229–236

    Article  PubMed  CAS  Google Scholar 

  11. Forbes C, O’Reilly C, McLaughlin L, Gilleran G, Tuohy M, Colleran E (2009) Application of high rate, high temperature anaerobic digestion to fungal thermozyme hydrolysates from carbohydrate wastes. Water Res 43:2531–2539

    Article  PubMed  CAS  Google Scholar 

  12. Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol 23:257–270

    Article  CAS  Google Scholar 

  13. Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59(2):257–268

    Article  CAS  Google Scholar 

  14. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  15. Bensadoun A, Weinstein D (1976) Assay of proteins in the presence of interfering materials. Anal Biochem 70:241–250

    Article  PubMed  CAS  Google Scholar 

  16. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  17. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Molecular Glycobiotechnology Research Group, Department of Biochemistry, School of Natural Sciences, National University of Ireland, University Road, Galway, Ireland

    Manimaran Ayyachamy & Maria G. Tuohy

  2. Department of Biochemistry, National University of Ireland, University Road, Galway, Ireland

    Mary Shier

Authors
  1. Manimaran Ayyachamy
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  2. Mary Shier
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  3. Maria G. Tuohy
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Corresponding author

Correspondence to Manimaran Ayyachamy .

Editor information

Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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Ayyachamy, M., Shier, M., Tuohy, M.G. (2013). Large-Scale Production of Lignocellulolytic Enzymes in Thermophilic Fungi. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_46

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