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Existence of Mesenchymal-Like Somatic Stem Cells in the Porcine Uterus

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Regenerative Medicine

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Abstract

Somatic stem cells are thought to be responsible for remarkable remodeling and regeneration of adult uterus. They are rare, undifferentiated cells capable of self-renewal and specialization into all endometrial and myometrial cellular components. So far best characterized by their functional properties, i.e., clonogenicity and ability to differentiate into four lineages (osteogenic, chondrogenic, adipogenic, myogenic) under appropriate in vitro conditions, are mesenchymal stem cells found primarily in the human and mouse and recently in porcine uterus. These cells are also defined by the expression of mesenchymal phenotypic markers (e.g., CD73, CD90, CD105) and lack of hematopoietic markers (CD34, CD45). Endometrial mesenchymal stem cells constitute a primary source for menstrual blood stem cells.

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References

  1. Chan RW, Schwab KE, Gargett CE. Clonogenicity of human endometrial epithelial and stromal cells. Biol Reprod. 2004;70:1738–50.

    Article  CAS  PubMed  Google Scholar 

  2. Gargett CE. Uterine stem cells: what is the evidence? Hum Reprod Update. 2007;13:87–101.

    Article  CAS  PubMed  Google Scholar 

  3. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143–7.

    Article  CAS  PubMed  Google Scholar 

  4. Casado JG, et al. Comparative phenotypic and molecular characterization of porcine mesenchymal stem cells from different sources for translational studies in a large animal model. Vet Immunol Immunopathol. 2012. http://dx.doi.org/10.1016/j.vetimm.2012.03.015.

  5. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Dj P, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy. 2006;8:315–7.

    Article  CAS  PubMed  Google Scholar 

  6. Gargett CE, Schwab KE, Zillwood RM, Nguyen HPT, Wu D. Isolation and culture of epithelial progenitors and mesenchymal stem cells from human endometrium. Biol Reprod. 2009;80:1136–45.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Maruyama T, Masuda H, Ono M, Kajitani T, Yoshimura Y. Human uterine stem/progenitor cells: their possible role in uterine physiology and pathology. Reproduction. 2010;140:11–22.

    Article  CAS  PubMed  Google Scholar 

  8. Kyo S, Maida Y, Inoue M. Stem cells in endometrium and endometrial cancer: accumulating evidence and unresolved questions. Cancer Lett. 2011;308:123–33.

    Article  CAS  PubMed  Google Scholar 

  9. Wolff EF, Wolff AB, Du H, Taylor HS. Demonstration of multipotent stem cells in the adult human endometrium by in vitro chondrogenesis. Reprod Sci. 2007;14:524–33.

    Article  CAS  PubMed  Google Scholar 

  10. Miernik K, Karasinski J. Porcine uterus contains a population of mesenchymal stem cells. Reproduction. 2012;143:203–9.

    Article  CAS  PubMed  Google Scholar 

  11. Ono M, Maruyama T, Masuda H, Kajitani T, Nagashima T, Arase T, Ito M, Ohta K, Uchida H, Asada H, et al. Side population in human uterine myometrium displays phenotypic and functional characteristics of myometrial stem cells. Proc Natl Acad Sci U S A. 2007;104:18700–5.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. Cervello I, Martinez-Conejero JA, Horcajadas JA, Pellicer A, Simon C. Identification, characterization and co-localization of label-retaining cell population in mouse endometrium with typical undifferentiated markers. Hum Reprod. 2007;22:45–51.

    Article  CAS  PubMed  Google Scholar 

  13. Donofrio G, Franceschi V, Capocefalo A, Cavirani S, Sheldon IM. Bovine endometrial stromal cells display osteogenic properties. Reprod Biol Endocrinol. 2008;6:65.

    Article  PubMed Central  PubMed  Google Scholar 

  14. Casal M, Haskins M. Large animal models and gene therapy. Eur J Hum Genet. 2006;14:266–72.

    Article  CAS  PubMed  Google Scholar 

  15. Gargett CE, Masuda H. Adult stem cells in the endometrium. Mol Hum Reprod. 2010;16:818–34.

    Article  CAS  PubMed  Google Scholar 

  16. Cervelló I, Gil-Sanchis C, Mas A, Delgado-Rosas F, Martínez-Conejero JA, Galán A, Martínez-Romero A, Martínez S, Navarro I, et al. Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells. PLoS One. 2010;5:e10964.

    Article  PubMed Central  PubMed  Google Scholar 

  17. Chan RW, Gargett CE. Identification of label-retaining cells in mouse endometrium. Stem Cells. 2006;24:1529–38.

    Article  CAS  PubMed  Google Scholar 

  18. Dimitrov R, Timeva T, Kyurkchiev D, Stamenova M, Shterev A, Kostova P, Zlatkov V, Kehayov I, Kyurkchiev S. Characterization of clonogenic stromal cells isolated from human endometrium. Reproduction. 2008;135:551–8.

    Article  CAS  PubMed  Google Scholar 

  19. Kato K, Yoshimoto M, Kato K, Adachi S, Yamayoshi A, Arima T, Asanoma K, Kyo S, Nakahata T, Wake N. Characterization of side-population cells in human normal endometrium. Hum Reprod. 2007;22:1214–23.

    Article  CAS  PubMed  Google Scholar 

  20. Hida N, Nishiyama N, Miyoshi S, Kira S, Segawa K, Uyama T, Moi T, Miyako K, Ikegami Y, Cui C, Kiyono T, Kyo S, Shimuzu T, Okano T, Sakamoto M, Ogawa S, Umezawa A. Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal. Stem Cells. 2008;26:1695–704.

    Article  CAS  PubMed  Google Scholar 

  21. Meng X, Ichim TE, Zhong J, Rogers A, Yin Z, Jackson J, Wang H, Ge W, Bogin V, Chan KW, Thébaud B, Riordan NH. Endometrial regenerative cells: a novel stem cell population. J Transl Med. 2007;5:57.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  22. Allickson JG, Sanchez A, Yefimenko N, Borlongan CV, Sanberg PR. Recent studies assessing the proliferative capability of a novel adult stem cell identified in menstrual blood. Open Stem Cell. 2011;3:4–10.

    Article  Google Scholar 

  23. Musina RA, Belyavski AV, Tarusova OV, Solovyova EV, Sukhikh GT. Endometrial mesenchymal stem cells isolated from the menstrual blood. Bull Exp Biol Med. 2008;14:539–43.

    Article  Google Scholar 

  24. Schwab KE, Chan RW, Gargett CE. Putative stem cell activity of human endometrial epithelial and stromal cells during the menstrual cycle. Fertil Steril. 2005;84:1124–30.

    Article  CAS  PubMed  Google Scholar 

  25. Masuda H, Matsuzaki Y, Hiratsu E, Ono M, Nagashima T, et al. Stem cell-like properties of the endometrial side population: implication in endometrial regeneration. PLoS One. 2010;5(4):e10387. doi:10.1371/journal.pone.0010387.

    Article  PubMed Central  PubMed  Google Scholar 

  26. Tsuji S, Yoshimoto M, Takahashi K, Noda Y, Nakahata T, Heike T. Side population cells contribute to the genesis of human endometrium. Fertil Steril. 2008;90:1528–37.

    Article  PubMed  Google Scholar 

  27. Wolff EF, Gao XB, Yao KV, Andrews ZB, Du H, Elsworth JD, Taylor HS. Endometrial stem cell transplantation restores dopamine production in a Parkinson’s disease model. J Cell Mol Med. 2011;15:747–55.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  28. Lynch L, Golden-Mason L, Eogan M, O’Herlihy C, O’Farrelly C. Cells with haematopoietic stem cell phenotype in adult human endometrium: relevance to infertility? Hum Reprod. 2007;22:919–26.

    Article  CAS  PubMed  Google Scholar 

  29. Onodera N, Tamaki T, Okada Y, Akatsuka A, Aoki D. Identification of tissue-specific vasculogenic cells originating from murine uterus. Histochem Cell Biol. 2006;125:625–35.

    Article  CAS  PubMed  Google Scholar 

  30. Wiese C, Rolletschek A, Kania G, Blyszczuk P, Tarasov KV, Tarasova Y, Wersto RP, Boheler KR, Wobus AM. Nestin expression – a property of multi-lineage progenitor cells? Cell Mol Life Sci. 2004;61:2510–22.

    Article  CAS  PubMed  Google Scholar 

  31. Wan Safwani WK, Makpol S, Sathapan S, Chua KH. The changes of stemness biomarkers expression in human adipose-derived stem cells during long-term manipulation. Biotechnol Appl Biochem. 2011;58:261–70.

    Article  PubMed  Google Scholar 

  32. Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Schöler H, Smith A. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor OCT4. Cell. 1998;95:379–91.

    Article  CAS  PubMed  Google Scholar 

  33. Matthai C, Horvat R, Noe M, Nagele F, Radjabi A, van Trotsenburg M, Huber J, Kolbus A. Oct-4 expression in human endometrium. Mol Hum Reprod. 2006;12:7–10.

    Article  CAS  PubMed  Google Scholar 

  34. Bentz EK, Kenning M, Schneeberger C, Kolbus A, Huber JC, Hefler LA, Tempfer CB. OCT-4 expression in follicular and luteal phase endometrium: a pilot study. Reprod Biol Endocrinol. 2010;8:38.

    Article  PubMed Central  PubMed  Google Scholar 

  35. Ono M, Kajitani T, Uchida H, Arase T, Oda H, Nishikawa-Uchida S, Masuda H, Nagashima T, Yoshimura Y, Maruyama T. OCT4 expression in human uterine myometrial stem/progenitor cells. Hum Reprod. 2010;25:2059–67.

    Article  CAS  PubMed  Google Scholar 

  36. Colter DC, Class R, DiGirolamo CM, Prockop DJ. Rapid expansion of recycling stem cells in cultures of plastic adherent cells from human bone marrow. Proc Natl Acad Sci U S A. 2000;97:3213–8.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  37. Deans RJ, Moseley AB. Mesenchymal stem cells biology and potential clinical uses. Exp Hematol. 2000;28:875–84.

    Article  CAS  PubMed  Google Scholar 

  38. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhajm P, Hedrick MH. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002;13:4279–95.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  39. Schwab KE, Gargett CE. Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium. Hum Reprod. 2007;22:2903–11.

    Article  CAS  PubMed  Google Scholar 

  40. Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med. 1996;183:1797–806.

    Article  CAS  PubMed  Google Scholar 

  41. Zhou S, Schuetz JD, Bunting KD, Colapietro AM, Sampath J, Morris JJ, Lagutina I, Grosveld GC, Osawa M, Nakauchi H, Sorrentino BP. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med. 2001;7:1028–34.

    Article  CAS  PubMed  Google Scholar 

  42. Taylor HS. Endometrial cells derived from donor stem cells in bone marrow transplant recipients. JAMA. 2004;292:81–5.

    Article  CAS  PubMed  Google Scholar 

  43. Du H, Taylor HS. Contribution of bone marrow-derived stem cells to endometrium and endometriosis. Stem Cells. 2007;25:2082–6.

    Article  CAS  PubMed  Google Scholar 

  44. Cervello I, Gil-Sanchis C, Mas A, Faus A, Sanz J, et al. Bone marrow-derived cells from male donors do not contribute to the endometrial side population of the recipient. PLoS One. 2012;7(1):e30260. doi:10.1371/journal.pone.0030260.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

The work was supported by the Ministry of Science and Higher Education Grant N N303804240.

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Authors and Affiliations

  1. Department of Cell Biology and Imaging, Institute of Zoology, Jagiellonian University, Gronostajowa 9, Krakow, 30-387, Poland

    Katarzyna Miernik MSc & Janusz Karasin´ski PhD

Authors
  1. Katarzyna Miernik MSc
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  2. Janusz Karasin´ski PhD
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Correspondence to Katarzyna Miernik MSc .

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Editors and Affiliations

  1. Calcutta School of Tropical Medicine, Kolkata, India

    Niranjan Bhattacharya

  2. Boston University School of Medicine, Jamaica Plain, Massachusetts, USA

    Phillip George Stubblefield

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Miernik, K., Karasin´ski, J. (2015). Existence of Mesenchymal-Like Somatic Stem Cells in the Porcine Uterus. In: Bhattacharya, N., Stubblefield, P. (eds) Regenerative Medicine. Springer, London. https://doi.org/10.1007/978-1-4471-6542-2_19

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  • DOI: https://doi.org/10.1007/978-1-4471-6542-2_19

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  • Print ISBN: 978-1-4471-6541-5

  • Online ISBN: 978-1-4471-6542-2

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