Skip to main content
SpringerLink
Log in

Characterizing the Synergistic Response Induced by Warm Temperatures and Shade

  • Protocol
  • First Online:
Thermomorphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2795))

  • 174 Accesses

Abstract

Plants exhibit an impressive capability to detect and respond to neighboring plants by closely monitoring changes in the light spectrum. They possess the ability to perceive adjustments in the ratio of red (R) to far-red (FR) light (R/FR) triggered by the presence of nearby plants, even before experiencing complete shading. When the R/FR ratio falls below 1, plants activate a shade avoidance response that manifests as hypocotyl elongation. Furthermore, elevated ambient temperatures can also stimulate hypocotyl elongation. As hypocotyl elongation is a visible characteristic, it is a valuable indicator for monitoring shade avoidance response, warm ambient temperature response, and the interplay between the two.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Bleecker AB, Estelle MA, Somerville C, Kende H (1988) Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana. Science 241(4869):1086–1089. https://doi.org/10.1126/science.241.4869.1086

    Article  CAS  PubMed  Google Scholar 

  2. Chory J, Peto C, Feinbaum R, Pratt L, Ausubel F (1989) Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light. Cell 58(5):991–999. https://doi.org/10.1016/0092-8674(89)90950-1

    Article  CAS  PubMed  Google Scholar 

  3. Deng XW, Caspar T, Quail PH (1991) cop1: a regulatory locus involved in light-controlled development and gene expression in Arabidopsis. Genes Dev 5(7):1172–1182. https://doi.org/10.1101/gad.5.7.1172

    Article  CAS  PubMed  Google Scholar 

  4. Estelle MA, Somerville C (1987) Auxin-resistant mutants of Arabidopsis thaliana with an altered morphology. Mol Gen Genet MGG 206(2):200–206. https://doi.org/10.1007/bf00333575

    Article  CAS  Google Scholar 

  5. Koornneef M, Rolff E, Spruit CJP (1980) Genetic control of light-inhibited hypocotyl elongation in Arabidopsis thaliana (L.). Heynh Zeitschrift für Pflanzenphysiologie 100(2):147–160. https://doi.org/10.1016/s0044-328x(80)80208-x

    Article  Google Scholar 

  6. Burko Y, Willige BC, Seluzicki A, Novák O, Ljung K, Chory J (2022) PIF7 is a master regulator of thermomorphogenesis in shade. Nature. Communications 13(1). https://doi.org/10.1038/s41467-022-32585-6

  7. Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez JY, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9(7):676–682. https://doi.org/10.1038/nmeth.2019

    Article  CAS  PubMed  Google Scholar 

  8. R Core Team (2022) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. https://www.R-project.org/

    Google Scholar 

  9. Wickham H (2016) ggplot2: elegant graphics for data analysis. Springer-Verlag, New York. ISBN 978-3-319-24277-4. https://ggplot2.tidyverse.org

    Book  Google Scholar 

  10. Mendiburu Fd (2020) Agricolae: Statistical Procedures for Agricultural Research. https://cran.r-project.org/package=agricolae

  11. Hope RM (2022) Rmisc. https://CRAN.R-project.org/package=Rmisc

  12. Clarke E, Sherrill-Mix S, Dawson S (2023) ggbeeswarm. https://CRAN.R-project.org/package=ggbeeswarm

  13. Spitzer M, Wildenhain J, Rappsilber J, Tyers M (2014) BoxPlotR: a web tool for generation of box plots. Nat Methods 11(2):121–122. https://doi.org/10.1038/nmeth.2811

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Espinosa-Ruiz A, Martínez C, Prat S (2015) Protocol to treat seedlings with Brassinazole and measure hypocotyl length in Arabidopsis thaliana. Bio-Protocol 5(16). https://doi.org/10.21769/BioProtoc.1568

Download references

Author information

Authors and Affiliations

  1. Department of Vegetables and Field Crops, Agricultural Research Organization, Volcani Institute, Bet Dagan, Israel

    Yogev Burko

Authors
  1. Yogev Burko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yogev Burko .

Editor information

Editors and Affiliations

  1. Department of Botany and Plant Sciences, Institute for Integrative Genome Biology, University of California, Riverside, CA, USA

    Meng Chen

Rights and permissions

Reprints and permissions

Copyright information

© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Burko, Y. (2024). Characterizing the Synergistic Response Induced by Warm Temperatures and Shade. In: Chen, M. (eds) Thermomorphogenesis. Methods in Molecular Biology, vol 2795. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3814-9_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-3814-9_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3813-2

  • Online ISBN: 978-1-0716-3814-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation