Abstract
The induction of oxidative stress by engineered nanomaterials has been associated with cytotoxic and inflammatory responses, damaging healthy cells and tissues. In contrast, when directed against cancer and autoinflammatory diseases, some nanomaterials inducing oxidative stress have also been reported as potential therapies for these disorders. Therefore, studying oxidative stress has become a popular tool not only in toxicology and immunotoxicology but in other areas of biology as well, including those related to developing novel therapies. Total oxidative stress may result from multiple cellular organelles. The protocol described herein allows for the analysis of oxidative stress in mitochondria.
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Acknowledgment
This project has been funded in whole or in part by federal funds from the National Cancer Institute, National Institutes of Health, under contract 75N91019D00024. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government.
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Shah, A., Dobrovolskaia, M.A. (2024). Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T Cells Using MitoSOX Red Dye. In: Clogston, J.D., Crist, R.M., Dobrovolskaia, M.A., Stern, S.T. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 2789. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3786-9_15
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DOI: https://doi.org/10.1007/978-1-0716-3786-9_15
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