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Urinary Bladder Cancer Induced by N-Butyl-N-(4-Hydroxybutyl)-Nitrosamine

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Mouse Models of Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2773))

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Abstract

Urinary bladder cancer is the tenth most common cancer worldwide with high morbidity and mortality. The majority of bladder cancers are urothelial carcinomas. More than half are papillomas or the papillary urothelial carcinomas (stages Ta and T1), which have a relatively good prognosis. Squamous cell carcinomas have a variable survival rate, while carcinomas in situ (Tis) can progress to muscle-invasive urothelial carcinomas (T2) with a poor prognosis. The most challenging feature of bladder cancer is its high recurrence rate, ranging from 50% to 90% of cases. The N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) model is an invaluable experimental tool for bladder cancer research, as BBN-induced bladder cancer in rodents resembles human bladder cancer in its morphological, biological, and molecular features. We present here a detailed protocol for the treatment of mice and the main expected results.

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Acknowledgments

The authors gratefully acknowledge financial support from the P3-0108, J7-2594, J3 2521, MRIC UL IP-0510 Infrastructure Programme of the Slovenian Research and Innovation Agency ARIS. We thank Sabina Železnik, Nada Pavlica Dubarić, Sanja Čabraja, and Linda Štrus for their technical support.

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Authors and Affiliations

  1. Faculty of Medicine, Institute of Cell Biology, University of Ljubljana, Ljubljana, Slovenia

    Daša Zupančič & Rok Romih

Authors
  1. Daša Zupančič
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  2. Rok Romih
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Correspondence to Rok Romih .

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Editors and Affiliations

  1. Institute of Oncology Ljubljana, Ljubljana, Slovenia

    Maja Čemažar

  2. Institute of Oncology Ljubljana, Ljubljana, Slovenia

    Tanja Jesenko

  3. Institute of Oncology Ljubljana, Ljubljana, Slovenia

    Urša Lampreht Tratar

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zupančič, D., Romih, R. (2024). Urinary Bladder Cancer Induced by N-Butyl-N-(4-Hydroxybutyl)-Nitrosamine. In: Čemažar, M., Jesenko, T., Lampreht Tratar, U. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 2773. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3714-2_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3714-2_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3713-5

  • Online ISBN: 978-1-0716-3714-2

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