Abstract
Radiotherapy is a widely used approach for cancer treatment. However, delivering a single high dose of radiation to bulky tumors can be challenging due to the toxicities induced in the surrounding healthy tissue. To overcome this issue, a nonuniform high dose can be delivered using partial-volume tumor irradiation or spatially fractionated radiotherapy (SFRT). Moreover, SFRT has the potential to induce a stronger antitumor immune response compared to traditional radiotherapy due to the preservation of immune cells in the unirradiated tumor regions. There are several SFRT approaches, including GRID therapy, three-dimensional GRID therapy (LATTICE), microbeam radiation therapy (MRT), and Stereotactic Body Radiation Therapy for PArtial Tumor irradiation targeting exclusively the HYpoxic segment (SBRT-PATHY). The following protocol describes partial-volume tumor irradiation, a technique that enables dose delivery to only a part of the tumor in mice using an X-ray generator and collimators of different dimensions that limit the size of the irradiation field.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Lim JYH, Gerber SA, Murphy SP et al (2014) Type I interferons induced by radiation therapy mediate recruitment and effector function of CD8+ T cells. Cancer Immunol Immunother 63:259–271
Demaria S, Formenti SC (2012) Radiation as an immunological adjuvant: current evidence on dose and fractionation. Front Oncol 2:1–7
Heylmann D, Rödel F, Kindler T et al (2014) Radiation sensitivity of human and murine peripheral blood lymphocytes, stem and progenitor cells. Biochim Biophys Acta 1846:121–129
Bogdándi EN, Balogh A, Felgyinszki N et al (2010) Effects of low-dose radiation on the immune system of mice after total-body irradiation. Radiat Res 174:480–489
Billena C, Khan AJ (2019) A current review of spatial fractionation: back to the future? Int J Radiat Oncol Biol Phys 104:177–187
Yan W, Khan MK, Wu X et al (2019) Spatially fractionated radiation therapy: history, present and the future. Clin Transl Radiat Oncol 20:30–38
Ha JK, Zhang G, Naqvi SA et al (2006) Feasibility of delivering grid therapy using a multileaf collimator. Med Phys 33:76–82
Wu X, Ahmed MM, Wright J et al (2010) On modern technical approaches of three-dimensional high-dose lattice radiotherapy (LRT). Cureus. https://doi.org/10.7759/cureus.9
Tubin S, Popper HH, Brcic L (2019) Novel stereotactic body radiation therapy (SBRT)-based partial tumor irradiation targeting hypoxic segment of bulky tumors (SBRT-PATHY): improvement of the radiotherapy outcome by exploiting the bystander and abscopal effects. Radiat Oncol 14. https://doi.org/10.1186/s13014-019-1227-y
Tubin S, Gupta S, Grusch M et al (2020) Shifting the immune-suppressive to predominant immune-stimulatory radiation effects by SBRT-PArtial Tumor Irradiation Targeting HYpoxic Segment (SBRT-PATHY). Cancers (Basel) 13:1–20
Fernandez-Palomo C, Fazzari J, Trappetti V et al (2020) Animal models in microbeam radiation therapy: a scoping review. Cancers 12. https://doi.org/10.3390/cancers12030527
Schültke E, Balosso J, Breslin T et al (2017) Microbeam radiation therapy – grid therapy and beyond: a clinical perspective. Br J Radiol 90:20170073. https://doi.org/10.1259/bjr.20170073
Markovsky E, Budhu S, Samstein RM et al (2019) An antitumor immune response is evoked by partial-volume single-dose radiation in 2 murine models. Int J Radiat Oncol Biol Phys 103:697–708
Rivera JN, Kierski TM, Kasoji SK et al (2020) Conventional dose rate spatially-fractionated radiation therapy (SFRT) treatment response and its association with dosimetric parameters – a preclinical study in a Fischer 344 rat model. PLoS One 15:e0229053. https://doi.org/10.1371/journal.pone.0229053
Acknowledgments
The authors acknowledge financial support from the Slovenian Research Agency under grant number P3-0003 and postgraduate research funding.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Modic, Z., Markelc, B., Jesenko, T. (2024). Partial-Volume Irradiation of Murine Tumors. In: Čemažar, M., Jesenko, T., Lampreht Tratar, U. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 2773. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3714-2_10
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3714-2_10
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3713-5
Online ISBN: 978-1-0716-3714-2
eBook Packages: Springer Protocols