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Culture-Independent Estimation of Lactic Acid Bacteria and Yeasts

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Basic Methods and Protocols on Sourdough

Part of the book series: Methods and Protocols in Food Science ((MPFS))

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Abstract

The population balances inside microbial consortia are highly variable from one sourdough ecosystem to another. In general, growth rate and yield of microorganisms are governed by a multitude of ecological factors. Indeed, in-depth studies of the biodiversity of the microbiota of traditional sourdough products are interesting from an academic and industrial point of view. Since not all sourdough microorganisms are easily cultivated in common laboratory media, modern molecular approaches such as the omics methodologies may help to further unravel sourdough fermentation processes. The application of metagenetics confirmed the dominance of species belonging to genera of Leuconostoc, Weissella, and ex-Lactobacillus for lactic acid bacteria and Saccharomyces and Kazachstania for yeasts. Here, detailed culture-independent methods commonly used to study the sourdough microbiota are described.

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Authors and Affiliations

  1. Department of Soil, Plant, and Food Science, University of Bari, Bari, Italy

    Erica Pontonio

  2. Department of Environmental Biology, Sapienza University of Rome, Rome, Italy

    Carlo Giuseppe Rizzello

Authors
  1. Erica Pontonio
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  2. Carlo Giuseppe Rizzello
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Correspondence to Erica Pontonio .

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Editors and Affiliations

  1. Faculty of Science and Technology, Free University of Bozen-Bolzano, BOLZANO, Bolzano, Italy

    Marco Gobbetti

  2. Department of Environmental Biology, Sapienza University of Rome, Rome, Italy

    Carlo Giuseppe Rizzello

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Pontonio, E., Rizzello, C.G. (2024). Culture-Independent Estimation of Lactic Acid Bacteria and Yeasts. In: Gobbetti, M., Rizzello, C.G. (eds) Basic Methods and Protocols on Sourdough. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3706-7_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3706-7_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3705-0

  • Online ISBN: 978-1-0716-3706-7

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