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Important Considerations for ELISpot Validation

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Handbook of ELISPOT

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2768))

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Abstract

The ELISpot assay has a solid place in the immune monitoring field for over 40 years. It is an assay that can assess the function of single immune cells in a straightforward and easy-to-learn approach. Its use in basic research, translational, and clinical work has been documented in countless publications. Harmonization guidelines and invaluable tools for optimal assay performance and evaluation exist. However, the validation of an established ELISpot protocol has been left to diverse opinions about how to interpret and tackle typical validation parameters. This chapter addresses important considerations for ELISpot validation, including the interpretations of validation parameters for a meaningful description of assay performance.

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Authors and Affiliations

  1. ZellNet Consulting, Inc., Fort Lee, NJ, USA

    Sylvia Janetzki

Authors
  1. Sylvia Janetzki
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Corresponding author

Correspondence to Sylvia Janetzki .

Editor information

Editors and Affiliations

  1. Biotechne, Minneapolis, MN, USA

    Alexander E. Kalyuzhny

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Janetzki, S. (2024). Important Considerations for ELISpot Validation. In: Kalyuzhny, A.E. (eds) Handbook of ELISPOT . Methods in Molecular Biology, vol 2768. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3690-9_1

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  • DOI: https://doi.org/10.1007/978-1-0716-3690-9_1

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3689-3

  • Online ISBN: 978-1-0716-3690-9

  • eBook Packages: Springer Protocols

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