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Supported Immunohistochemical Staining of Mucins

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Mucins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2763))

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Abstract

Immunohistochemistry (IHC) staining is the most common method to detect the distribution and localization of biomarkers in different parts of a tissue. Antibodies for tandem repeat peptide of mucins are very popular, but antibodies for glycosylation or others are also used. IHC for mucin is the same protocol as IHC for others. This description includes IHC according to ABC method for processed formalin-fixed paraffin-embedded (FFPE) tissues. Protocol of in situ hybridization is also shown.

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References

  1. Cartum RW, Taylor CR, Dabbs DJ (2021) Techniques of immunohistochemistry. In: Dabbs DJ (ed) Diagnostic immunohistochemistry, 6th edn. Saunders Elsevier, Philadelphia

    Google Scholar 

  2. https://vectorlabs.com

  3. https://www.abcam.com

  4. https://www.agilent.com/ja-jp/product/immunohistochemistry

  5. https://www.roche.com/

  6. Yonezawa S, Sueyoshi K, Nomoto M et al (1997) MUC2 gene expression is found in noninvasive tumors but not in invasive tumors of the pancreas and liver: its close relationship with prognosis of the patients. Hum Pathol 28:344–352

    Article  PubMed  Google Scholar 

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (KAKENHI 21 K06889) from the Japan Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Department of Pathology, Field of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan

    Michiyo Higashi

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  1. Michiyo Higashi
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Correspondence to Michiyo Higashi .

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Editors and Affiliations

  1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Akihiko Kameyama

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Higashi, M. (2024). Supported Immunohistochemical Staining of Mucins. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3670-1_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3669-5

  • Online ISBN: 978-1-0716-3670-1

  • eBook Packages: Springer Protocols

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