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Structural Elucidation of Sialylated O-Glycan Alditols Obtained from Mucins by Mass Spectrometry

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Mucins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2763))

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Abstract

Acidic O-glycans having sialic acid and/or sulfate residue are abundantly expressed in intestinal mucins. However, structural elucidation of acidic O-glycans is a laborious and time-consuming task due to their large structural variations. Here, we describe a methodology of structural elucidation for sialylated O-glycan alditols from intestinal mucins using tandem mass spectroscopy. Methylesterification and mild periodate oxidation of sialylated O-glycan alditols assist mass analysis. This description includes the purification process of O-glycan alditols for structural analysis.

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Acknowledgements

The authors express our sincere appreciation to S. Sugawara for her technical assistance.

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Authors and Affiliations

  1. Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Japan

    Daigo Tsubokawa

  2. Department of Biochemistry, Kitasato University School of Allied Health Science, Sagamihara, Japan

    Rei Kawashima & Takafumi Ichikawa

Authors
  1. Daigo Tsubokawa
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  2. Rei Kawashima
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  3. Takafumi Ichikawa
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Corresponding author

Correspondence to Takafumi Ichikawa .

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Editors and Affiliations

  1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Akihiko Kameyama

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Tsubokawa, D., Kawashima, R., Ichikawa, T. (2024). Structural Elucidation of Sialylated O-Glycan Alditols Obtained from Mucins by Mass Spectrometry. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_18

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  • DOI: https://doi.org/10.1007/978-1-0716-3670-1_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3669-5

  • Online ISBN: 978-1-0716-3670-1

  • eBook Packages: Springer Protocols

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