Abstract
Mucin glycomic analysis is crucial owing to the participation of mucin O-glycans in several biological functions. Liquid chromatographic analysis of fluorescently labeled glycans is an effective tool for glycomic analysis. The first step of this analysis involves the release of O-glycans from mucins. As no enzyme is known to release all glycans, chemical methods are required for the process; therefore, hydrazine treatment is a commonly used chemical method. It enables the release of O-glycans from mucin while preserving the aldehyde group at the reducing end. This ensures that the reducing end can be modified using fluorescent reagents. However, it is also accompanied by the degradation of the glycans through a process called “peeling.” Here, we describe a method for releasing glycans from mucins using hydrazine treatment with minimal “peeling.”
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Acknowledgments
This work was supported by the Japan Society for the Promotion of Science KAKENHI grant 25460934.
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Goso, Y., Kurihara, M. (2024). Preparation of O-Glycans from Mucins Using Hydrazine Treatment. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_12
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DOI: https://doi.org/10.1007/978-1-0716-3670-1_12
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3670-1
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