Abstract
MS-target analyses are frequently utilized to analyze and validate structural changes of biomolecules across diverse fields of study such as proteomics, glycoproteomics, glycomics, lipidomics, and metabolomics. Targeted studies are commonly conducted using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) techniques. A reliable glycoproteomics analysis in intricate biological matrices is possible with these techniques, which streamline the analytical workflow, lower background interference, and enhance selectivity and specificity.
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Acknowledgments
Access to Exploris 240 mass spectrometer was provided by Prof. Luis Herrera-Estrella, President’s Distinguished Professor of Plant Genomics and Director of the Institute of Genomics for Crop Abiotic Stress Tolerance at Texas Tech University. This work is supported by grants from the National Institutes of Health, NIH, including 1R01GM112490-08, 1R01GM130091-05, and 1U01CA225753-05. The work is also supported by grants from Robert A. Welch Foundation (No. D-0005) and The CH Foundation.
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Gutierrez Reyes, C.D. et al. (2024). Targeted Glycoproteomics Analysis Using MRM/PRM Approaches. In: Bradfute, S.B. (eds) Recombinant Glycoproteins. Methods in Molecular Biology, vol 2762. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3666-4_14
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DOI: https://doi.org/10.1007/978-1-0716-3666-4_14
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