Abstract
Chayote (Sechium edule) belongs to the Cucurbitaceae family, an important family at the nutritional and medicinal levels, that has been covering international markets. Having vigorous and healthy plants is important for producers, who are very interested in cultivating chayote plants obtained from in vitro tissue culture in their orchards. Bioreactors have become an alternative with high potential for plant propagation, showing significant advantages over micropropagation in semisolid medium, by generating more plant material, larger, and more vigorous. In this chapter, a micropropagation protocol of S. edule in RITA® bioreactors is reported.
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References
Cadena-Iñiguez J (2010) El chayote (Sechium edule (Jacq.) SW.), importante recurso fitogenético mesoamericano. Agroproductividad 3:3–11
Vieira E, Pinhob O, Ferreira I, Delerue C (2019) Chayote (Sechium edule): a review of nutritional composition, bioactivities and potential applications. Food Chem 257:557–568. https://doi.org/10.1016/j.foodchem.2018.09.146
Salazar-Aguilar S, Ruiz-Posadas LDM, Cadena-Iñiguez J, Soto-Hernández M, Santiago-Osorio E, Aguiñiga-Sánchez I, Rivera-Martínez AR, Aguirre-Medina JF (2017) Sechium edule (Jacq.) Swartz, a new cultivar with antiproliferative potential in a human cervical cancer HeLa cell line. Nutrients 9:798. https://doi.org/10.3390/nu9080798
Veigas GJ, Bhattacharjee A, Hegde K, Shabaraya AR (2020) A brief review on Sechium edule. Int J Pharm Sci Rev Res 65:165–168. https://doi.org/10.47583/ijpsrr.2020.v65i02.026
Soto-Contreras A, Núñez-Pastrana R, Rodríguez-Deméneghi M, Aguilar-Rivera N, Galindo-Tovar M, Ramírez-Mosqueda M (2022) Indirect organogenesis of Sechium edule (Jacq.) Swartz. In Vitro Cell Dev Biol Plant 58:903–910. https://doi.org/10.1007/s11627-022-10304-6
Etienne H, Berthouly M (2002) Temporary immersion systems in plant micropropagation. Plant Cell Tissue Cult 69:215–231. https://doi.org/10.1023/A:1015668610465
Georgiev V, Schumann A, Pavlov A, Bley T (2014) Temporary immersion systems in plant biotechnology. Eng Life Sci 14:607–621. https://doi.org/10.1002/elsc.201300166
Steingroewer J, Bley T, Georgiev V, Ivanov I, Lenk F, Marchev A, Pavlov A (2013) Bioprocessing of differentiated plant in vitro systems. Eng Life Sci 13:26–38. https://doi.org/10.1002/elsc.201100226
Mirzabe AH, Hajiahmad A, Fadavi A, Rafiee S (2022) Temporary immersion systems (TISs): a comprehensive review. J Biotechnol 357:56–83. https://doi.org/10.1016/j.jbiotec.2022.08.003
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Cruz-Martínez V, Castellanos-Hernández OA, Acevedo-Hernández GJ, Torres-Morán MI, Gutiérrez-Lomelí M, Ruvalcaba-Ruiz D, Rodríguez-Sahagún A (2017) Genetic fidelity assessment in plants of Sechium edule regenerated via organogenesis. S Afr J Bot 112:118–122. https://doi.org/10.1016/j.sajb.2017.05.020
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Ramírez-Trejo, L., Núñez-Pastrana, R., Soto-Contreras, A. (2024). Micropropagation of Chayote (Sechium edule L.) var. virens levis in RITA®. In: Ramírez-Mosqueda, M.A., Cruz-Cruz, C.A. (eds) Micropropagation Methods in Temporary Immersion Systems. Methods in Molecular Biology, vol 2759. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3654-1_10
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DOI: https://doi.org/10.1007/978-1-0716-3654-1_10
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