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Investigations on Microbes Attached to the Cuticle of Phytonematodes

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Plant-Nematode Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2756))

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Abstract

Nematodes form various associations with soil microbiome. Experimental studies on nematode-attached microbes can improve mechanistic understanding of these associations and lead to new discoveries relevant for the field of nematode biocontrol. Microbial attachment to the surface of phytonematodes is very specific and influenced by a multitude of factors, including the designation of nematodes and microbes, environmental and biological factors in soil, time of incubation, and the ratio and evolutionary trajectories between nematodes and microbes. Here, we describe how the classical nematological and microbiological techniques can be coupled with the advanced molecular tools to study the microbial attachment to phytonematodes in soil. We focus on the characterization of nematode-attached microbes using classical microbiological approaches and high-throughput amplicon sequencing and on the effects of nematode-attached microbes on plant defense responses.

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Authors and Affiliations

  1. Department of Biology, Section of Terrestrial Ecology, University of Copenhagen, Copenhagen, Denmark

    Olivera Topalović

  2. Laboratory of Insect and Nematode Management, Corteva Agriscience Research, Center Eschbach, Eschbach, Germany

    Zoran S. Radaković

  3. Epidemiology and Pathogen Diagnostics, Julius Kuehn Institute, Braunschweig, Germany

    Ahmed Elhady & Holger Heuer

  4. Rheinische Friedrich-Wilhelms-University of Bonn, INRES—Molecular Phytomedicine, Bonn, Germany

    Sandra Bredenbruch

Authors
  1. Olivera Topalović
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  2. Zoran S. Radaković
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  3. Ahmed Elhady
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  4. Sandra Bredenbruch
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  5. Holger Heuer
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Corresponding author

Correspondence to Olivera Topalović .

Editor information

Editors and Affiliations

  1. IPSP, Consiglio Nazionale delle Ricerche CNR, Bari, Italy

    Sergio Molinari

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Topalović, O., Radaković, Z.S., Elhady, A., Bredenbruch, S., Heuer, H. (2024). Investigations on Microbes Attached to the Cuticle of Phytonematodes. In: Molinari, S. (eds) Plant-Nematode Interactions. Methods in Molecular Biology, vol 2756. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3638-1_7

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  • DOI: https://doi.org/10.1007/978-1-0716-3638-1_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3637-4

  • Online ISBN: 978-1-0716-3638-1

  • eBook Packages: Springer Protocols

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