Abstract
An analysis of the mitochondrial respiration function represented by the oxygen consumption rate is necessary to assess mitochondrial bioenergetics and redox function. This protocol describes two alternative techniques to evaluate mitochondrial respiration function in situ: (1) measure oxygen consumption rates via an electrode; (2) measure oxygen consumption rates via a seahorse instrument. These in situ approaches provide more physiological access to mitochondria to evaluate mitochondrial respiration function in a relatively integrated cellular system.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (81970333) and the National Key Research and Development Program of China (2018YFA0107102).
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Li, A., Qin, Y., Zhang, Y., Zhen, X., Gong, G. (2024). Evaluation of Oxygen Consumption Rates In Situ. In: Gilkes, D.M. (eds) Hypoxia. Methods in Molecular Biology, vol 2755. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3633-6_16
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DOI: https://doi.org/10.1007/978-1-0716-3633-6_16
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3632-9
Online ISBN: 978-1-0716-3633-6
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