Skip to main content
SpringerLink
Log in

Serum Western Blot for the Detection of a c-Myc Protein Tag in Non-human Primates and Mice

  • Protocol
  • First Online:
Alpha-1 Antitrypsin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2750))

  • 237 Accesses

Abstract

This protocol allows for the detection of a c-Myc tag on alpha-1 antitrypsin (AAT) delivered to species that already have endogenous AAT such as non-human primates allowing reliable and repeatable semi-quantitation of serum levels of AAT.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Gruntman AM, Flotte TR (2015) Progress with recombinant adeno-associated virus vectors for gene therapy of alpha-1 antitrypsin deficiency. Hum Gene Ther Methods 26(3):77–81. https://doi.org/10.1089/hgtb.2015.086

    Article  PubMed  PubMed Central  Google Scholar 

  2. Pires Ferreira D, Gruntman AM, Flotte TR (2023) Gene therapy for alpha-1 antitrypsin deficiency: an update. Expert Opin Biol Ther 23(3):283–291. https://doi.org/10.1080/14712598.2023.2183771

    Article  PubMed  Google Scholar 

  3. Gruntman AM, Flotte TR (2017) Therapeutics: gene therapy for alpha-1 antitrypsin deficiency. Methods Mol Biol 1639:267–275. https://doi.org/10.1007/978-1-4939-7163-3_27

    Article  PubMed  Google Scholar 

  4. Borel F, Sun H, Zieger M, Cox A, Cardozo B, Li W, Oliveira G, Davis A, Gruntman A, Flotte TR, Brodsky MH, Hoffman AM, Elmallah MK, Mueller C (2018) Editing out five Serpina1 paralogs to create a mouse model of genetic emphysema. Proc Natl Acad Sci U S A 115(11):2788–2793. https://doi.org/10.1073/pnas.1713689115

    Article  PubMed  PubMed Central  Google Scholar 

  5. Tang Q, Gruntman AM, Flotte TR (2017) Quantification of Total human alpha-1 antitrypsin by sandwich ELISA. Methods Mol Biol 1639:211–216. https://doi.org/10.1007/978-1-4939-7163-3_20

    Article  PubMed  Google Scholar 

  6. Gruntman AM, Gernoux G, Tang Q, Ye G-J, Knop DR, Wang G, Benson J, Coleman KE, Keeler AM, Mueller C, Chicoine LG, Chulay JD, Flotte TR (2019) Bridging from intramuscular to limb perfusion delivery of rAAV: optimization in a non-human primate study. Mol Ther Methods Clin Dev 13:233–242. https://doi.org/10.1016/j.omtm.2019.01.013

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatrics and Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA

    Meghan Blackwood, Qiushi Tang & Alisha M. Gruntman

Authors
  1. Meghan Blackwood
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qiushi Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alisha M. Gruntman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alisha M. Gruntman .

Editor information

Editors and Affiliations

  1. Alpha-1 Biologics, Long Island High Technology Incubator, Stony Brook University, Stony Brook, NY, USA

    Cynthia L. Bristow

Rights and permissions

Reprints and permissions

Copyright information

© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Blackwood, M., Tang, Q., Gruntman, A.M. (2024). Serum Western Blot for the Detection of a c-Myc Protein Tag in Non-human Primates and Mice. In: Bristow, C.L. (eds) Alpha-1 Antitrypsin. Methods in Molecular Biology, vol 2750. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3605-3_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-3605-3_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3604-6

  • Online ISBN: 978-1-0716-3605-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation