Abstract
Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell–cancer cell interactions, such as cytolysis analysis using xCELLigence technology.
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Rahbech, A., Debets, R., thor Straten, P., Peeters, M.J.W. (2024). Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis. In: Siciliano, V., Ceroni, F. (eds) Cancer Immunotherapy. Methods in Molecular Biology, vol 2748. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3593-3_3
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DOI: https://doi.org/10.1007/978-1-0716-3593-3_3
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Online ISBN: 978-1-0716-3593-3
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