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Preparation and Characterization of Collagen–Hyaluronic Acid (Col–HA) Matrices: In Vitro Mimics of the Tumor Microenvironment

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Proteases and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2747))

Abstract

Collagen-I (Col) and hyaluronic acid (HA) are two of the most important extracellular matrix (ECM) components. While collagen serves as the main structural feature of the ECM and is an important ligand for integrin-based adhesions, HA acts as a ligand for the transmembrane glycoprotein CD44. However, most studies on cancer invasion utilize 3D collagen matrices, thereby ignoring the importance of HA–CD44 interactions. Here, we describe the process of fabricating Col–HA gels with varying Col/HA ratios, which can be used to suitably mimic the tumor microenvironment.

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Authors and Affiliations

  1. Department of Biosciences & Bioengineering, IIT Bombay, Mumbai, India

    Sarbajeet Dutta & Shamik Sen

Authors
  1. Sarbajeet Dutta
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  2. Shamik Sen
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Corresponding author

Correspondence to Shamik Sen .

Editor information

Editors and Affiliations

  1. Department of Biochemical Sciences, School of Biosciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, UK

    Salvatore Santamaria

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Dutta, S., Sen, S. (2024). Preparation and Characterization of Collagen–Hyaluronic Acid (Col–HA) Matrices: In Vitro Mimics of the Tumor Microenvironment. In: Santamaria, S. (eds) Proteases and Cancer. Methods in Molecular Biology, vol 2747. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3589-6_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3589-6_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3588-9

  • Online ISBN: 978-1-0716-3589-6

  • eBook Packages: Springer Protocols

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