Abstract
Intracellular cytokine staining is a versatile technique used to analyze cytokine production in individual cells by flow cytometry. This methodology has the specific advantage of enabling the simultaneous assessment of multiple phenotypic, differentiation, and functional parameters pertaining to responding T cells. This methodology applied after short-term culture of cells, followed by fixation and permeabilization make this technique ideal for the assessment of T-cell immune responses induced by different challenges. Here we describe an intracellular staining method followed by flow cytometry after cell stimulation with immune-relevant antigens for Lyme disease.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Calderilla-Barbosa, L., Flores-Sevilla, J.L. (2024). CYTOSPOT: Intracellular Staining and Detection of Cytokines by Flow Cytometer, an Alternative to LYMESPOT. In: Gilbert, L. (eds) Borrelia burgdorferi. Methods in Molecular Biology, vol 2742. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3561-2_7
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DOI: https://doi.org/10.1007/978-1-0716-3561-2_7
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3561-2
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