Abstract
Cell cycle control is a central aspect of the biology of proliferating eukaryotic cells. However, progression through the cell cycle relies on a highly complex network, making it difficult to unravel the core design principles underlying the mechanisms that sustain cell proliferation and the ways in which they interact with other cellular pathways. In this context, the use of a synthetic approach to simplify the cell cycle network in unicellular genetic models such as fission yeast has opened the door to studying the biology of proliferating cells from unique perspectives. Here, we provide a series of methods based on a minimal cell cycle module in the fission yeast Schizosaccharomyces pombe that allows for an unprecedented artificial control of cell cycle events, enabling the rewiring and remodeling of cell cycle progression.
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References
Morgan DO (1997) Cyclin-dependent kinases: engines, clocks, and microprocessors. Annu Rev Cell Dev Biol 13:261–291
Morgan DO (2007) The cell cycle: principles of control. New Science Press, London
Coudreuse D, Nurse P (2010) Driving the cell cycle with a minimal CDK control network. Nature 468:1074–1079
Perrot A, Millington CL, Gómez-Escoda B et al (2018) CDK activity provides temporal and quantitative cues for organizing genome duplication. PLoS Genet 14:e1007214
Swaffer MP, Jones AW, Flynn HR et al (2016) CDK substrate phosphorylation and ordering the cell cycle. Cell 167:1750–1761.e16
Gutiérrez-Escribano P, Nurse P (2015) A single cyclin–CDK complex is sufficient for both mitotic and meiotic progression in fission yeast. Nat Commun 6:6871
Banyai G, Baïdi F, Coudreuse D et al (2016) Cdk1 activity acts as a quantitative platform for coordinating cell cycle progression with periodic transcription. Nat Commun 7:11161
Chen T, Gomez-Escoda B, Munoz-Garcia J et al (2016) A drug-compatible and temperature-controlled microfluidic device for live-cell imaging. Open Biol 6:160156
Moreno S, Hayles J, Nurse P (1989) Regulation of p34cdc2 protein kinase during mitosis. Cell 58:361–372
Moreno S, Klar A, Nurse P (1991) [56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 194:795–823
Gérard C, Tyson JJ, Coudreuse D et al (2015) Cell cycle control by a minimal Cdk network. PLoS Comput Biol 11:e1004056
Stern B, Nurse P (1996) A quantitative model for the cdc2 control of S phase and mitosis in fission yeast. Trends Genet 12:345–350
Fisher DL, Nurse P (1996) A single fission yeast mitotic cyclin B p34cdc2 kinase promotes both S-phase and mitosis in the absence of G1 cyclins. EMBO J 15:850–860
Bishop AC, Ubersax JA, Petsch DT et al (2000) A chemical switch for inhibitor-sensitive alleles of any protein kinase. Nature 407:395–401
Uemura T, Yanagida M (1984) Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization. EMBO J 3:1737–1744
Watt S, Mata J, López-Maury L et al (2008) urg1: a uracil-regulatable promoter system for fission yeast with short induction and repression times. PLoS One 3:e1428
Yamano H, Gannon J, Hunt T (1996) The role of proteolysis in cell cycle progression in Schizosaccharomyces pombe. EMBO J 15:5268–5279
Nurse P, Thuriaux P, Nasmyth K (1976) Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 146:167–178
Acknowledgments
This work was supported by grants to DC from the Agence Nationale de la Recherche (PRC eVOLve, ANR-18-CD13-0009), the Région Nouvelle Aquitaine (program CHESS, grant agreements 15963520 and 15964420), and the Ligue Contre le Cancer (Gironde, Dordogne). AJ was funded by the ANR eVOLve. PYW was supported by the Région Nouvelle Aquitaine (program CHESS, grant agreements 15963520 and 15964420) and the Ligue Contre le Cancer (Pyrénées-Atlantiques).
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Jain, A., Wu, PY.J., Coudreuse, D. (2024). Artificial Modulation and Rewiring of Cell Cycle Progression Using Synthetic Circuits in Fission Yeast. In: Castro, A., Lacroix, B. (eds) Cell Cycle Control. Methods in Molecular Biology, vol 2740. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3557-5_5
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DOI: https://doi.org/10.1007/978-1-0716-3557-5_5
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